Lab Methodologies

Absorbance spectrophotometry

measures the concentration (of the amount of light absorbed) of an analyte using Beer's Law

Nephelometry

measures the light scatter of macromolecule complexes

Enzyme Immunoassay

Uses a nonisotopic label such as horseradish peroxidase + measures antibodies/antigens based on the assay

Immunofluorescence

uses antibody labeled with fluorescein isothiocyanate (FITC) + for antibody-antigen complexes

FISH

a molecular cytogenetic technique using fluorescence + Fluorescence in situ hybridization

PCR

amplifies the target region of DNA using oligonucleotide primers

Coulometry

measures the amount of current passing between two electrodes in an electrochemical cell

Electrophoresis

uses the principle of different molecules having different velocities in an electrical field (migration of molecules across an electrical field

Chromatography

separates mixtures of solutes dissolved in a common solvent by a differential distribution of the solutes between two phases

Flow cytometry

uses light scatter to identify cells