Blood banking (from book)

What is the purpose of washed red cells?

Prevent allergic response

Purpose of leukocyte reduction

Prevents febrile nonhemolytic reactions, HLA alloimmunization, cmv transmission

Purpose of irradiated blood?

Prevents GVHD, inactivates T cells

H + Le?

H, Lea RBCs, Lea saliva

H + Le + Se?

H, Leb RBCs, H, Lea, Leb saliva

How to fix a rouleaux formation?

Saline wash

Purpose of “check cells” in IAT?

check negative results, should give positive reaction

Which ab are enhanced by enzymes?

Kidd, I, P1, Lewis, Rh

Which abs are destroyed by enzyme treatment?

Duffy, MNSs

Which abs show dosage?

Rh, M, M, JKa, JKb, Fya, Fyb

How does a DAT show a positive result?

AHG is added and agglutinates

Kleihauer-Belk calculation?

(fetal cells/ 2000 × 5000) / 30. round + 1