unit 4 - serology

serology

study of blood serum containing antibodies

serological tests

based on very specific antibody-antigen interactions, as antibodies are very specific as to what antigens they bind to

positive vs negative results in serological tests

positive = ab/ag binding

negative = no ab/ag binding

serotypes

bacterial strains with different antigens

list serology tests

1. slide agglutination test

2. ELISA - enzyme-linked immunosorbent assay

• direct ELISA (detecting antigens)

• indirect ELISA (detecting antibodies)

slide agglutination tests

testing for cell clumping by antibodies

ELISA (enzyme-linked immunosorbent assay)

use of specific antibodies to detect pathogen presence (Antigen) or exposure to a pathogen (antibody)

direct ELISA

• what it detects for

• what it uses to detect it

• application

detects antigen using known antibody

applied via adding drug or hormone to urine

use a diagram with labels to show the process of detecting a positive sample using direct ELISA

detects antigens

use a diagram with labels to show the process of detecting a positive sample using indirect ELISA

detects antibodies