Bloodbanking Quiz 3.5

Alloantibody definition

foreign ab, rare

Antigram definition

antigen profile of screening cells, cell map

Autoantibody definition

antibodies made by self to self cells

Antibody panel purpose

identifies specificity of rbc antibodies

Antibody screen purpose

detects antibodies with specificity to rbc antigens

Autocontrol composition

patient serum and cells

Clinically significant definition

hdn, htr, decrease survival of rbcs

Phase of reaction definition

phase where agglutination occurs

Dosage definition

homozygous reacts stronger than heterozygous

Potentiator definition

enhancement media used to increase speed/specificity of ab/ag attachment

Types of patients who require ab screening

transfusion recipients, surgical/obstetric patients, donor units

Principle of antibody screening

detect slinically significant abs in pt serum w/ known screening cells

Procedure for antibody screening

immediate spin, potentiator, incubation, ahg, ccc

Preferred specimen for antibody screening

fresh, pink edta, avoid hemolysis (false pos)

Information obtained from IS phase

Lewis, MN, I P

Information obtained from 37C phase

Rh

Information obtained from AHG phase

kell, kidd, duffy

Purpose of autocontrol

checks integrity of patient cells and autoantibodies

Follow up procedure for positive antibody screen

review patient history, antibody panel

Purpose of elution in antibody ID

remove IgG attached to RBCs in vivo, perform panel on eluate

What to review in panel reactions

phase of reaction, reaction strength

Purpose of analyzing reaction strength in panel

indicate number of antibodies present, dosage

3 Aspects of ruling out

panel cells that give negative reactions in all phases can be used to rule out abs, matching patterns, rule of three

Matching patterns in ruling out

observe cells for similar patterns to agglutination

Rule of three in ruling out

three antigen positive panel cells react and three antigen negative panel cells don’t react

Purpose of select cells

additional cells used when there are multiple potential antibodies

What to do after antibody ID

phenotype the patient’s red cell for the antigen to the specific antibody, should be negative

Techniques to deactivate cold antibodies

prewarming and autoadsorption