12 - Methods of biochemical analysis II

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Last updated 3:11 PM on 6/2/26
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7 Terms

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Immunoaffinity chromatography

antibody against protein of interest  

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Immoblised ligand chromatography

substrate analogue or inhibitor binds enzyme or protein

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Lectin-based affinity chromatography

lectin bind glycosylated proteins; elution is via addition of sugar

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Immobilised metal affinity chromatography

metal ions bind engineered recombinant proteins containing a poly-histidine tag at the N- or C- terminus.  

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SDS-PAGE

used to estimate molecular weight of a protein

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SDS treatment ensured proteins are :

separated on the basis of their size / charge is irrelevant

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At neutral pH, SDS forms___________with polypeptide chains in ____________forms.

Complexes / denatured