7.1 Gene Expression in Eukaryotes

What does DNAase stand for?

Deoxyribonuclease 

How are nucleosomes ‘cleared’ from active chromatin?

Sliding / eviction

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Non-polar globular domain

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N-terminal variable basic arm

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C-terminal variable basic arm 

What are histone arms consisting of?

Lysines

What kind of biochemical molecule in lysine?

Amino acid

What is the charge on lysine?

Positive

What gives DNA, its -ve charge?

The phosphates

What are the 3 post-translation modifications of histones?

Methylation / Acetylation / phosphorylation 

What is methylation?

-CH3 groups added to lys and his 

What is acetylation?

Addition of CH3CO2- group to lysines of core histones

What is phosphorylation?

Addition of PO4- to lysines of H1

What does methylation do?

Gene activation and repression, DNA replication

What does acetylation do?

Nucleosome assembly: gene activation 

What does phosphorylation modification of histone do?

Cell division / transcription / Chromatin structure