enterobac test ID: lec test2

TSI

contains glucose lactose and sucrose, and ferric ammonium, A/A= all sugar ferment, K/A= only glucose fermented, K/K=no sug ferment

urea

detects urease, turns urea into ammonia

citrate

determines organism uses citrate as its own carbon source (produce alkaline byproduct)

decarboxylase

detects enzymes that remove carbox grp (produce co2=alk byproduct)

what are the common AA tested for in decarbox test and what are their end products

lysine to cadaverine, ornathine to putrescine, arginine to ornathine

deamination (test) -TDA

detects whether AA can be deaminated by removing AA group (ferric chloride) TDA=tryptophan

indole

detects tryptophanase (break down tryptophan and indole is byproduct) Kovacs and erlich reagent (p-dimethylaminobenxaldehyde)

voges proskauer (VP)

detects fermnetation of flucose through the butanediol ferment. pathway acetoin is final product -reagents; KOH, and alpha naphthol

methyl red (MR)

metabolize glucose through mixed acid ferment. produces pyruvate which gets metabolized to acid

nitrate to nitrite

detects nitrates can be reduced to nitrates (anaerobic resp) -reagents after bath: sulfanic acid N.N dimethyl alpha naphthylamine then add zinc

ONPG

detects beta galactosidase enzyme (lactose ferment) rapid v slow ferment

motility

detects motility; TTC (tetrazolium salts) indicator

oxidase

detects cytochrome C oxidase enzyme (aerobic resp) ONLY PLEISIOMONAS

what are the antigens on some Enterobacterales organisms

O: somatic, part of LPS, heat stable, serotyping (protein); H: flagellar, heat labile; K: capsular, heat labile; Vi: capsular virulence antigen (salmonella)

what are the virulence factors in Enterobacterales

LPS and pili

what plates can they ve found on

BAP, choc, MAC, SS, Hektoen , XLD, GN broth, and selenite broth

what kind of bac are enterobac

facultative anaerobes

what are enterobac optimal growth temperatures

37-37 deg C