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ATP can be synthesized in 2 ways:
Substrate-level Phosphorylation
Chemiosmosis
Substrate-level Phosphorylation
An enzyme takes in a substrate, combines it with a phosphate group to react with ADP. The finished product and ATP are released
Chemiosmosis
The working on the mitochondrial inner membrane to produce ATP through ATP synthase
Glucose goes through
the different metabolic pathways to produce ATP

Oxidative Phosphorylation
Oxidative Phosphorylation
ATP formation associated with the flow of electrons through the respiratory/ electron transport chain
Electron Transport Chain (ETC):
Series of protein complexes and carriers in mitochondrial inner membrane that pass electrons from NADH and FADH2 to Oxygen. Using that energy to pump in Protons (H+)
In the ETC….
Water is the end product
Oxygen is the final electron acceptor
NADH/FADH2 feeds into the ETC, and…
2.5 ATP are produced
What happens in the absence of Oxygen?
Fermentation
Fermentation…
happens in the cytoplasm. It regenerates NAD+ to keep glycolysis going