Nucleic Acid & Purine/Pyrimidine Tests

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Last updated 10:54 AM on 9/11/26
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4 Terms

1
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Dische (Diphenylamine)

Target Analyte: Deoxyribose (DNA)

Reagents / Principle: Diphenylamine in acidic conditions.

Positive Result: Blue solution. (RNA/ribose gives a green color).

2
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Murexide

Target Analyte: Purines (Adenine, Guanine, Uric Acid)

Reagents / Principle: Nitric acid oxidation followed by the addition of ammonia.

Positive Result: Purple-red (murexide) residue.

3
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Ammonium Molybdate

Target Analyte: Phosphates (Nucleic acids, phospholipids)

Reagents / Principle: Ammonium molybdate in nitric acid.

Positive Result: Yellow crystalline precipitate (ammonium phosphomolybdate).

4
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Knoop / Wheeler-Johnson

Target Analyte: Pyrimidines (Cytosine, Uracil)

Reagents / Principle: Bromine water oxidation followed by barium hydroxide Ba(OH)2.

Positive Result: Green or Purple color/precipitate.