lab 4 aseptic technique and proper handling of microorganisms

pipette

an instrument often used to transfer aliquots of culture, to prepare serial dilutions of microorganisms, and to dispense chemical reagents

blowout pipette (serological pipette)

to deliver pipette (mohr measuring pipette)

two types of measuring pipette frequently used

blowout pipette

pipette use with final few drops of liquids must be empty again in order to deliver the correct volume

to deliver pipette

pipette that is used when after the proper amount of liquid has been delivered, liquid will remain in the tip of the pipette and should not be eliminated

inoculating needles and loops

used to aseptically transfer microorganisms from broth, slant, or agar cultures to other media

inoculating needles and loops

consists of the handles, a shaft, and a turret, which holds a nickel chromium or platinum wire

aseptic technique

crucial in characterizing an unknown organism

aseptic technique

an obligatory in isolating and purifying bacteria from a mixed source of organisms

streak plate and pour plate

provides a means to isolate an individual species