Category 1A Protein Structure and Function

Gel Electrophoresis

Separation of proteins based primarily on chage, size, and shape

SDS-PAGE

Proteins are first denatured with SDS. This eliminates protein shape differences and gives them all a negative charge eliminating charge differents. Therefore it separates proteins based on size differences,

Isoelectric Focusing

Electrophoresis performed on a gel with a pH gradient. A protein migrates to the point in the gel where the pH = pI

What are the 3 types of columb chromatography?

Ion exchange, size exclusion, affinity chromatography.

What is the difference between SDS page and Native page when separating proteins?

SDS denatures the proteins so it only separates them based on size

Native does not denature so separation is based on size, shape, and charge