fall micro week 1

homogenization

tissue grinding

capillary

blood sample by pricking skin, sterile

central line or PICC

blood drawn from peripherally inserted catheter

catheter

collects urine from bladder not sterile

syprapubic

aspiration from bladder via needle, sterile

peritoneal

fluid lining abdominal organs, sterile

sterile spec

fluid, bm, abcess, tissue, wound

MAC

grows gram neg, inhib gram pos

PEA and CNA

grow gram pos, inhib gram neg

msa

grows staphylooccus, inhib others

LIM broth

enriched, encourage growth of small numbers of a particular org while suppressing others

THIO broth

with growth on agar plates to detect small numbers of most aerobes, anaerobes and microaerophiles

isolation tech

inoculate non selective media then selective, then streak

aerobe vs anaerobe vs facilitative anaerobes

oxygen, no oxygen to grow, better with but can without

psychrophiles, mesophiles, thermophiles

10-20 C few path, 20-45 C human path and most bac, 50-200 few path

QC documentation

kept up to 2 years, done day of use

GPC transmission and virulence

in foormites, dirt, dust, floors, walls, spreads by direct contact, toxins and enzymes causing inflammation

staphlococcus

18-24 hrs for growth, non motile, nonspore forming, opaque smooth circular, gray-white yellow, beta in aureus and hemolyticus

catalase

3% h2o2, pos bubbles for staph and micrococcus, cannot use BAP bc rbcs contain pseudo cat giving false pos

coagulase on slide vs tube

fibrinogen to fibrin after mixed with plasma, vs staphylocoagulase reacting with CRF takes 4 hrs

fibrinolysin

produced by some s aureus, dissolves the clot

rapid latex agglu

staphaurex, staphTEX, plasma coated latex, fibrinogen detects clumping factor, IgG detects protein A, very spec/sens, done with nonselective plates

novobiocin

Resistant for staph saprophyticus, growth less than 16mm

pyr hydrolysis

L-pyrrolidonyl-naphthylamide with free B-naphthlamide and DMAC, pos red staph lugdunensis

ornithine decarboxylase

2-4hrs, modified moeller ornithine, pos purple S lugdunensis

GPC flow

catalase pos staph, coag pos aureus, neg do micodase pos micrococcus, neg novobiocin R staph sap

micrococcus tests

microdase- DMSO pos purple in 30 sec micrococcus, bacitracin S no growth greater than 10 mm, glucose use- yellow only with O2 micrococcus

s. epidermidis

immunocomp or implant device biofilm, pink on MSA

s. lugdunesis

delayed beta, white, pyr pos, ornithine pos

alpha on SBA flow

optochin S= strep pneumo, R do bile esculin neg virdians, pos do 6.5% nacl pyr pos enterococcus neg group D

beta hemolytic colonies flow

bacitracin S group A pyogenes, R do CAMP pos group B agalactiae, neg probs D

gamma flow

bile esculin pos do 6.5 nacl pos enterococcis pyr pos, neg group D pyr neg

bile solubility

clear pos for strep pneumo

hippurate hydrolysis

pos purple group B strep

lactose fermentors on MAC

e coli, kleb, enterobacter, citrobacter freundii

SS agar

pink e coli, black salmolella

HE agar

blue-green and black salmonella, black proteus

XLD

yellow e coli, yellow and black proteus, colorless red-pink shigella, red and black salmonella

oxidase

pos pseudomonas, neg enterobacteriacae

indole pos

e coli, kleb oxy, proteus vulg, citro koseri, morg morg, aeromonas

carb utilization

both yellow e coli, open yellow pseudomonas oxidizer

TSI

K/A bubble black salmonella, A/A bubbles e coli, K/K psedomonas, K/A shigella

ONPG

pos yellow ecoli and enterobacter

MR vs VP

red e coli vs e. cloacae

citrate

pos blue salmonella, kleb pneu

motile

e coli, enterobacter, citrobacter, salmonella, serratia