Bio Lab Exam 1

Cell Counter Slide

Slide

Transfer Pipette

Cover Slip

Micropipette

Forceps

Micropipette tip

Erlenmeyer Flask

Microcentrifuge Tube

Beaker

Ocular Lens

Rotating Nose Piece

Objective Lens

Arm

Mechanical Stage

Stage

Fine Adjustment Knob

Coarse Adjustment Knob

Stage Adjustment Knob

Base

Substage Light (Light Source)

Iris Diaphragm of Condenser

Cleaning the Objective lenses, Eye Piece, and Condenser using

Lens Paper

Clean the Stage using

Kimwipe

Clean Oil using

Lens Cleaner

After use the stage should be moved to?

Lowest Level

After use the microscope is set to

Scanning Lens (4x Objective Lens)

Total Magnification =

Ocular Magnification (10x) X Objective Magnification (4x, 10x, 40x, 100x)

Which Dye was used on the Cheek Cells

Methylene Blue

How does the colored thread slide demonstrate depth of field?

Colored thread slides showed depth of field because different layers of thread come into focus at different times. At higher magnification, fewer threads came into focus.

How does the colored thread slide demonstrate field of view?

Colored Thread slides demonstrates field of view because different amounts of the thread can be seen at different magnifications. At high magnification, less of the thread can be seen

As the stage adjustment turned right

The Stage is moved to the left

Things seen through the microscope are

Vertically Inverted

Closterium (Algae)

Volvox (Colonial, Algae)

Scenedesmus (Colonial, Algae)

Name and Locomotion

Amoeba, Pseudopodia (Protist, Phagocytosis)

Name and Locomotion

Paramecium, Cilia (Protist, Vacuoles)

Name and Locomotion

Euglena, Flagella (Protist, Eye Spot, Photosynthesis).

What should always be labeled when posting a specimen observed under a microscope?

Identification of the specimen, and total magnification.

Spirillum

Single Bacillus

Streptobacillus

Staphylococcus

Tetracoccus

Diplococcus

Single Coccus

Prokaryotic Cells: Bacteria and Archaea

Generally smaller in size compared to eukaryotic cells, no membrane bound organelles, less complex, outer layer is the cell wall.

Eukaryotic Cells: Plants, Fungi, Protists, and Animal cells

Generally larger than prokaryotic cells, it has membrane bound organelles, more complex, and the outer layer is the cell membrane

What to do with a broken glass slide or cover slip?

Use proper tools to dispose of it in the glass disposal box

Why should a micropipette always be left upright?

Avoids contamination, loss of accuracy, and internal damage

Which dye is used for cell counting?

Trypan Blue, Nonpolar.

Which dye is used for staining cheek cells?

Methylene Blue (Lipophilic)

Control Flask

A flask that does not receive the experimental treatment and is used as a comparison.

Experimental Flask

A flask that receives the experimental treatment to observe its effects.

Independent Variable

The Factor that is manipulated in the experiment.

Z?

Control Flask (pH 7.8)

A?

Experimental Flask (Acidic pH 5.0)

R?

The indication of replication.

What is Replication?

Repeating an experiment multiple times under the same conditions to ensure the results are consistent and reliable.

The research question?

How does an acidic environment affect the growth and characteristics of Chlorella vulgaris algae?

H₀ Null Hypothesis

The hypothesis that supports there being no effect or relationship between the independent and dependent variables.

H₁ Alternative Hypothesis

The Hypothesis that supports there being a relationship between the independent and dependent variables.

What is the Independent Variable in our Experiment?

The pH of the solution the algae grows in.

What is the Dependent Variable in our Experiment?

The growth and change in Chlorella vulgaris Algae Characteristics

What is the Control Variable in our Experiment?

Factors kept the same in our experiment, amount of light, temperature, and initial amount of algae.

What is the Scientific Method?

A step-by-step process scientists use to ask questions, tests hypothesis, and draw conclusions based on results.

Scientific Method Process?

Observation, Form a question, Form a Hypothesis, Make Predictions, Experiment, Analyze results, form conclusion, repeat processes and communicate results.

If one molecule has a higher molecular weight (Methylene Blue), compared to one with a lower molecular weight (Potassium Permanganate), which will diffuse faster?

The molecule with a lower molecular weight will diffuse faster