DNA Manipulating enzymes

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Last updated 5:34 AM on 10/16/26
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19 Terms

1
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Basic step in gene cloning

-cut & slip gene into a vector

-slip into a bacterium so it can multiply

2
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gene cloning process


3
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DNA manipulation


4
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What joins DNA strand and what joins nucleotide?


5
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Nuclease


6
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Ligase


7
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Polymerase


8
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Types of polymerase reactions


9
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DNA Modifying enzyme


10
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DNA Structure


11
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Why is DNA negatively charged?


12
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why it matters for DNA to be -


13
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Restriction endonuclease


14
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Sticky vs Blunt end


15
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Compare sticky and blunt end


16
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Types of restriction endonucleases


17
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why precision matters?


18
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Discovery of restriction endonuclease


19
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How often does an enzyme cut ?