Lab 6: Protista - Amoebozoa

Kingdom protista are always…

• single-celled organisms

• with an encapsulated nucleus

What is one way to distinguish between protists?

motility structure

What is the motility structure of amoebas?

pseudopodia

What domain are amoebas in?

eukarya

What is the generalized life cycle of amoebozoa?

• trophozoites in host

• trophozoites → cyst passed in feces

• cysts in environment

• cyst is ingested

How do you get infected with Entamoeba histolytica?

from any kind of drinking water contaminated with fecal matter

What is the best way to filter water in a pinch?

iodine crystals

What are the steps in the life cycle of Entamoeba histolytica?

• ingest the cyst

• cyst goes to intestinal tract and becomes a trophozoite

• trophozoite feeds on intestines and ingests nutrients

• trophozoites clone each other

• trophozoites go back to cysts and exit in feces

trophozoite

feeding/moving/harming stage that lives inside the host

cyst

transmission stage that leaves and enters the host

Which amoeba only has a trophozoite stage?

Entamoeba gingivalis

Does Entamoeba gingivalis have a cyst stage?

no, there’s no environmental part of the life cycle, just person to person

How is Entamoeba gingivalis transmitted?

through kissing (exchanging saliva)

Is Naegleria fowleri naturally a parasite?

no, it is naturally free-living

Where does Naegleria fowleri live?

at the bottom of freshwater lakes

What is the only amoeba that has 3 different stages, and what are they?

Naegleria fowleri;

cyst, trophozoite, and flagellate

What is the transmission stage of Naegleria fowleri?

trophozoite

How is Naegleria fowleri transmitted?

by getting water with the trophozoite up your nose;

the trophozoite latches onto sinus mucosa and crawls up the nose to the brain and starts eating the brain

Can you get infected by Naegleria fowleri by drinking the transmission stage?

no

Which amoeba infection can you get by misusing a Netipot?

Naegleria fowleri