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Cell
the basic structural and functional units of every organism
All Cells …
are bound by a plasma membrane
contain cytosol
contain chromosomes
contain ribosomes
Plasma/cell Membrane
a thin barrier that surrounds every living cell and separates its inside from the outside world
Prokaryotes
no nucleus or membrane-bound organelles
Eukaryotes
Nucleus + membrane bound organelles
Ribosome
synthesize proteins
translate messages found on mRNA into the primary structure of polypeptides
Chromosome
DNA itself gets coiled and wrapped around proteins to form the chromosome.
Nucleoid
membrane-free region inside a prokaryotic cell that holds all or most of the genetic material
Nucleus
stores and protects DNA in eukaryotic cells
Organelle
membrane-bound structures in eukaryotes
Endomembrane System
a network of membranes and organelles in eukaryotic cells that work together to modify, package, and transport polysaccharides lipids and proteins
Vesicle
membrane-bound bubble inside a cell that moves proteins, nutrients, or waste from one part of the cell to another
mitochondria
produces energy for the cell called ATP
Site of cellular respiration
Double Membrane - outer membrane is smooth, inner membrane is highly convoluted (many folds called cristae)
Energy Organelles
covert energy to forms that cells can use for work
Types of Energy Organelles:
Mitochondria
Chloroplasts
Endomembrane Organelles
Nuclear envelope
Endoplasmic reticulum
Golgi complex
Lysosmes
vesicles/vacuoles
Plasma membrane
Cytosol
water-based fluid inside a cell where the organelles float
Compartmentalization
Eukaryotic membranes and membrane-bound organelles allow diff. metabolic processes and enzymatic reactions occur in different locations
effects of compartmentalization:
Increases surface area of reactions to occur
Prevents intefering reactions from occuring in the same location
Chloroplast
site of photosynthesis
contains the green pigment chlorophyll
Nuclear Envelope outer trait
double membrane
has pores (to regulate entry and exit of materials fro mthe cell
Continuous with the ER membrane
Nucleolus (what is it, what happens there?)
Dense region of the nucleus where ribosomal rRNA is synthesized
In the nucleolus, rRNA combines with proteins to form large and small subunits of ribosomes
How are ribosomes formed?
In the nucleolus, rRNA combines with proteins to form large and small subunits of ribosomes
subunits of ribosomes exit via nuclear pores and assemble into ribosomes
Endoplasmic Reticulum
A network of membranous sacs (cisternae) and tubules
helps cells maintain shape
intracellular transport
produces, folds, and modifies biological molecules, then ships them inside tiny vesicles to where they are needed
Types of ER
Rough ER:
contains ribosomes bound to the ER membrane
makes + transports proteins
Smooth ER:
Contains no ribosomes
makes lipids and detoxifies the cell
Where is the Ribosome found?
Cytosol
Bound to the ER or nuclear envelope
Cytosol
Proteins produced her generally function only within the cytosol (i.e. enzymes)
known as “free ribosomes”
Ribosomes bound to ER or nuclear envelope can do what?
proteins produced here can leave the cell via transport vesicles
Golgi Complex
receives transport vesicles with materials from the ER
modifies and sorts the materials
adds molecular tags
packages materials into new transport vesicles that exit the membrane.
Cisternae
Membranous sacs found in the ER and golgi complex
Directionality of Golgi complex
Cis face: receives vesicles from the ER
Trans face: sends vesicles back out into the cytosol to other locations or to the plasma membrane
Lyosomes
membranous sac with hydrolyte enzymes in animal cells
created by cisternae
hydrolyzes macromolecules
Autphagy: recycle the cell’s materials and organelles that are not functioning properly
allows the cell to renew itself
plays a role in apoptosis (programmed cell death)
Apoptosis ( programmed cell death)
membrane becomes permeable —> releases enzymes into cytoplasm —> breaks down cellular components
Peroxisomes
membrane bound metabolic compartment
catalyze reactions that produce H2O2
break down fatty acid molecules, creates certain phospholipids, detoxify compounds
Enzyme in peroxisomes break H2O2 into water and oxygen
H2O2
can detoxify certain compounds and damage the cell if any escapes
Vacuoles
Vesicles that stem from the ER and golgi
seletive in transport
Difference in vacuoles in animal and plant cells
Animal Cells:
small in size
assist endoctysosis/exocytosis processes
store cellular materials
Plant Cells:
Central vacuole, large in size
stores nutrients and water
can function like lysosomes
Plasmodesmata
channels passing through the cell walls of plants that allow direct communication and transport between cells
Centrosomes
organelle that helps cell division and organises cytoskeleton
Flagella
helps make the cell move
central vacuole
stores water and supports the structural system of plant cells
Unique Cell Components in Plants and Animals
Plants:
chloroplasts
central vacuole
cell wall
plasmodesmata
Animas:
lysosomes
centrosomes
flagella
Role of Cristae in the mitochondria
divides the mitochondria into two internal compartments and increases the surface area
Intermembrane
space between inner and outer membrane
mitochondrial matrix
enclosed by inner membrane
location for the krebs cycle
contains enzymes, mitochondrial dna, and ribosomes
Mitochondria + activity?
the number of mitochondria in a cell correlates with metabolic activity
cells with high metabolic activity ahve more mitochondria
Inside the double membrane of chloroplast
Thylakoids and stroma
Thylakoids
membranous sacs that can organize into stacks called grana
light dependent reactions occur in grana
Stroma
fluid around thylakoids
location for calvin cycle
contains: chloroplast DNA, ribosomes, and enzymes
Endosymbiotic Theory
mitochondria and chloroplasts evolved from once free - living prokaryotic cells
became endosymbiont and one functional organism
Evidence: 1. microtubules
microfilaments
intermediate filaments
Microtubules
thickest filaments in cytoskeleton
in animal cells they grow from the centrosome
structural support for the movement of organelles that interact with proteins
assist the movement of chromosomes during cell division
cell motility (the ability of cells to move)
Microfilaments
Thin, solid rods made of protcein actin
maintain cell shape
assist in muscle contraction and cell motility
division of animal cells
Intermediate filaments
Fibrous proteins made up of varying subunits
permanent structural elements of cells
maintain cell shape
anchor nucleus and organelles
form the nuclear lamina (lines the nuclear envelope)
centricoles
structured within a centrosome, helps with nuclear division and composed of microtubules.
tight junctions
join cells together in the formation of tissues
middle lamella
first layer of the cell wall