BIO-221 Lab Practical #1

Which of the following lists containment controls?

Safety equipment, laboratory practices, and facility construction

A facility laboratory…

• handles nonpathogenic microbes

• has open lab benches

• requires personal protective equipment as needed

• has sink for hand washing

• doors to separate lab from rest of facility

Which of the following BSLs is described?

BSL-1

A facility laboratory…

• handles indigenous microbes

• has restricted access to lab

• has biological safety cabinets and autoclaves

• requires personal protective equipment

• includes an eye wash

• has self-closing doors

Which of the following BSLs is described?

BSL-2

A facility laboratory…

• handles indigenous or exotic microbes

• has employees under medical surveillance

• has restricted and controlled access to lab

• has work done in a biological safety cabinets

• requires personal protective equipment

• has a hands-free sink and eye wash

• has 2 self-closing and locking doors

• where exhaust air is NOT recirculated

Which of the following BSLs is described?

BSL-3

A facility laboratory…

• handles dangerous and exotic microbes

• requires changing of clothes before entering

• materials are decontaminated before exiting

• requires showering upon exiting

• uses Class III biological safety cabinet or a positive pressure suit

• may be a separate building or in a restricted zone of a building

• has a dedicated vacuum line and decontamination system

Which of the following BSLs is described?

BSL-4

Penicillium conidiophore size

2.5 micrometers

Saccharomyces size

5 micrometers

Spirogyra size

Width of 80 micrometers and length of 170 micronmeters

Volvox size

325 micrometers

Coprinus size

7,325 micrometers

Rank volvox, coprinus, saccharomyces, spirogyra, and penicillium from smallest to largest.

Penicillium, saccharomyces, spirogyra, volvox, and coprinus

Function of immersion oil

Increases resolving power of the microscope at high magnification

How does immersion oil enhance magnification?

Catches light and focuses light into the objective lens

Which of these lists the purpose of a capsule?

Surface attachment & protection from predation and unfavorable environmental factors

Why is the capsule stain a negative stain?

The capsule isn’t stained, only the background is.

Purpose of an endospore

A survival structure for when a cell is undergoing adverse environmental conditions

What purpose does steam serve in an endospore stain?

Traps malachite green in the endspore

Which of the following CANNOT be seen with the results of an endospore stain?

The new cell forming inside an endospore

What purpose does steam serve in an acid-fast stain?

Softens the mycolic acids, allowing carbolufuschin to dye the cell.

Why are petri dishes plates incubated upside down?

To prevent condensation from forming on the agar

In a gram positive cell, why does the cell appear purple?

The thick peptidoglycan wall retains the purple after the slide is washed with Gram’s Decolorizer and water

In a gram negative cell, why does the cell appear show red?

The thin peptidoglycan appears red from the safranin because the crystal violet is washed off from the water and Gram’s Decolorizer