Bio 265L Lab Week #6 Review

These flashcards cover key concepts and test procedures related to Staphylococcus, Streptococcus, and Enterococcus species as discussed in lab week #6.

Mannitol Salt Agar (MSA) plate

A selective medium used to differentiate Staphylococcus species based on their ability to ferment mannitol.

Beta hemolysis

A type of hemolysis where bacteria completely lyse red blood cells, turning the medium clear.

Coagulase activity

A test to determine if a species like Staphylococcus aureus produces an enzyme that coagulates plasma.

BAP (Blood Agar Plate)

A non-selective differential medium used to identify hemolytic activity of bacteria.

CAMP test

A test used to identify Streptococcus agalactiae (Group B strep) based on its interaction with Staphylococcus aureus.

Bile Esculin Test

A selective/differential medium used to identify Enterococcus species by their positive reaction that changes the color of the medium.

Alpha hemolysis

A type of hemolysis where bacteria partially lyse red blood cells, resulting in a greenish discoloration of the medium.

Optochin sensitivity test

A test used to differentiate Streptococcus pneumoniae (sensitive) from other alpha-hemolytic streptococci (resistant).

Staphylococcus epidermidis

A coagulase-negative staphylococcus, typically non-hemolytic (gamma hemolysis) on BAP.

Streptococcus pyogenes

A Group A beta-hemolytic streptococcus, sensitive to bacitracin.

Streptococcus agalactiae

A Group B beta-hemolytic streptococcus, resistant to bacitracin and positive for CAMP factor.