QUESTIONS for EXERCISE 10 HYDROLYSIS OF LIPID, STARCH, and CASEIN

what is the substrate for starch hydrolysis

starch

what is the enzyme that catalyzes starch

amylase

what is the process happening during starch hydrolysis

hydrolysis: splitting starch molecules into simple sugars by breaking covalent bonds with addition of water

what products are produced in starch hydrolysis

simple sugars

how do you visualize starch hydrolysis / what reagent must be added to visualize it

add Gram’s iodine

what does positive starch hydrolysis look like

cleared zone of orange/yellow color next to growth

what does negative starch hydrolysis look like

purple black color where starch remains undigested

what is the substrate for lipid hydrolysis

lipid (fat)

what enzyme catalyzes lipids

lipase

what products are produced in lipid hydrolysis

glycerol and fatty acids

what process happens during lipid hydrolysis

cleaving lipid molecules into smaller components

how do you visualize lipid hydrolysis / what reagent must be added to visualze it

no reagent needed to be added, Spirit Blue indicator dye is in the agar plate

what does a positive lipid hydrolysis test look like

cleared zones indicating oil digestion (could also see dark blue precipitate from free fatty acids reacting with spirit blue)

what does a negative lipid hydrolysis look like

no clearing of the oil from the agar

what is the substrate for casein hydrolysis

casein (the principle protein in milk)

what enzyme catalyzes casein

caseinase

what process is happening during casein hydrolysis

hydrolyzing proteins into smaller units

what products are produced in casein hydrolysis

peptides and amino acids

how do u visualize casein hydrolysis / what reagent must be added to visualize

no reagent added, visually examine milk

what does positive casein hydrolysis look like

cleared zones around streaks

what does negative casein hydrolysis look like

no clearing, the milk agar remains opaque