Bio2924 - Lab Midterm

The purpose of lab safety rules and protocols is to

protect everyone in the lab from injury and infection.

Which of the following are appropriate times to clean and disinfect the laboratory bench?

-Between each step of the lab protocol if the lab instructions call for this procedure.

-After the completion of lab activities.

-Prior to the start of lab activities.

Slides, scalpels, razor blades, cover glasses, and capillary tubes are all classified as

sharps

The lab shower is used to clean large spills within the fume hood

false

If you sustain a small cut in the lab that can readily be covered with a Band-Aid, there is no need to notify the lab instructor.

false

Which of the following are appropriate times to complete hand washing?

-Between each step of the lab protocol if the lab instructions call for this procedure.

-Any time the hands are potentially contaminated with chemicals or microorganisms.

-After the completion of lab activities.

-Prior to the start of lab activities.

Which of the following materials should be discarded in a biohazard bag?

-Plastic petri dishes with cultures

-Dissection tissue

-Disinfectant-soaked paper towels used to clean a microbial spill.

Personal protective equipment (PPE) can help prevent contamination of an experiment.

true

Which of the following materials should be discarded in the item pictured here?

-Glass slide and cover glass

-Scalpel blades

Scrap notes, paper towels, and lens paper should be discarded into a _____ bin when you are finished with them.

trash

Which of the following materials should be washed in the sink?

-Tools used in preserved specimen dissection.

-Tray used in preserved specimen dissection.

-Used glass beaker

After they are used, reusable instruments or glassware that require sterilization should be placed in the

autoclave

All laboratory chemicals can be disposed of by pouring them down the drain.

false

Which of the following statements concerning lab protocols is correct?

Make-up and contact lenses should never be applied during lab.

Which of the following materials should be discarded in a biohazard bag or biohazard bin?

-Long plastic serological pipettes

-Dissection tissue

-Plastic petri dishes with cultures

Which of the following are appropriate types of shoes in the lab?

Tennis shoes

Boots

Which of the following are potential hazards encountered in the laboratory?

-Open flames

-Corrosive chemicals

-Sharps instruments

-Infectious agents

Your hands are a potential source of contamination for some experiments.

true

You have finished working with some bacterial cultures in glass tubes. What should you do to dispose of these cultures?

autoclave them

Information on the hazards posed by a chemical used in the laboratory can consistently be found

on the Safety Data Sheets (SDS).

Which of the following selections below are laboratory safety protocols established to protect?

-Visitors to the lab who do not understand the science experiment.

-The lab workers directly involved with the protocol.

-The lab equipment and materials used in the protocol.

-Immunocompromised individuals who enter into biohazardous lab rooms.

When you are finished with the dissection tray and forceps used in dissection of a preserved specimen you should

wash in the sink

Which of the following materials should be washed in the sink?

-Beaker used for steaming during a staining exercise.

-Forceps and probe used in dissection of a preserved specimen.

In which of the following situations would you use the fume hood pictured here?

Working with hazardous chemicals.

Dissection specimens and bacterial culture plates should be discarded into a _____ bin when you are finished with them.

biohazard

Infectious agents in the laboratory are classified into four _____ levels based on their ease of transmission and ability to cause disease.

biosafety

Which of the following should be done first in the event of a laboratory accident?

Notify the instructor.

Hand washing is not necessary if gloves have been worn throughout the lab exercise.

false

The disinfectant spray in this image is most appropriately used for

cleaning up infectious agents.

Which of the following are examples of personal protective equipment (PPE)?

-Gloves

-Lab coat

Which of these items can be placed in the regular trash can or recycle container?

Scrap notes that are no longer needed, which were used during a lab and did not contain dangerous chemicals or infectious organisms.

Which of the following items must be placed into a hard-sided sharps container rather than a biohazard bag or regular trash can?

-Glass slide and cover glass

-Pasteur pipettes (glass)

-Scalpel blades

-Needles/syringes

Which of the following are personal items on the laboratory bench?

-Phone

-Purse

-Book bag

The goal of aseptic technique is to

-prevent contamination of cultures in the microbiology lab.

-prevent the establishment of an infectious agent in the host.

Can you be certain that you made a successful transfer if your sterile broth is turbid after incubation?