Booodbanking Must know (PART 2)

4 hours (2-4 hours)

BLOOD TRANSFUSION

The administration of blood or its component intravenously is done within:

Every 16 weeks

Frequency of red cell pheresis donation

• Every 48 hrs

• Not more than 24 times a year

Frequency of plateletpheresis donation

Every 4 weeks

Frequency of plasmapheresis donation

• Not more than twice a week

• Not more than 24 times a year

Frequency of leukopheresis donation

AHTR

Reaction due to clinically significant antibody reacting at 37 degC

FNHTR

Reaction is due to patient’s anti-leukocyte antibody

TRALI

Reaction is due to leukoagglutinins

Yersinia enterocolitica

Most common bacteria associated with Transfusion Associated Sepsis

TACO

Associated with rapid infusion of large volumes of blood products

IFA or Western blot

Confirmatory test for HIV-1

RIBA

Confirmatory test for Hepatitis C

RIPA

Confirmatory test for Trypanosoma cruzi

• anti-Lea

• Leb

• PP1Pk

• Vel

In vitro hemolysis are seen with which antibodies?

IgM

Which type of antibody reacts during IS phase and is usually clinically insignificant?

• Ficin

• Papain

• Bromelin

• Trypsin

Proteolytic enzymes

Urine

Source of Anti-Sda

Breast milk

Source of Anti-I

Major Crossmatch

PS + DR

Minor Crossmatch

DS + PR

Broad spectrum

Most preferred type of crossmatch

Tube method

It is the gold standard technique for crossmatching

1 mL

In HDFN, as little as __ mL can immunize the mother

D

It is the most immunogenic of all antigens

Anti-Kell

Considered the most clinically significant antibody in its ability to cause HDFN

Erythroblastosis fetalis

Term used to describe the stimulation of fetal bone marrow to produce RBCs at an accelerated rate due to destruction of fetal RBCs

Hydrops fetalis

Term used to describe the development of high output cardiac failure with generalized edema, effusions, and ascites caused by severe anemia and hypoproteinemia

Cordocentesis

The procedure used to obtain a sample of fetal blood

Amniocentesis

• For management of HDFN

• Used to estimate the extent of fetal hemolysis

10 g/dL

The goal of Intrauterine Transfusion is to maintain fetal hemoglobin above :

Phototherapy

It is used to change the unconjugated bilirubin to isomers which are less lipophilic and less toxic to the brain

Exchange Transfusion

Employs the use of whole blood or equivalent to replace the neonate’s circulating blood

• Prevents kernicterus

Anemia (requires transfusion)

EXCHANGE TRANSFUSION

Below 10 g/dL

Severe anemia

EXCHANGE TRANSFUSION

Below 7 g/dL

DAT

It is the most important serologic test for HDFN diagnosis

300 ug

RhIG dose which protects against 15 mL of packed RBCs or 30 mL of whole blood

Kleihauer-Betke

Test to quantitate actual amount of fetomaternal hemorrhage