BIO 1301- Agar, specs, pathogens

Culture media used for throat spec

BA or SSA

Culture media used for eye (conjuctiva)

BA

CA

GS

Culture media for middle ear spec

BA

MAC

CA

GS

Culture media for sputum, bronchial washes, and other lower respiratory specs

BA

MAC

CA

GS

Culture media for urine

BA

MAC

PEA (if spec is from nephrostomy)

GS (optional)

Culture media for CSF

BA

CA

MAC (if gram - rod seen on direct GS)

Thio (if CSF obtained via shunt)

GS

Culture media for superficial wound or abscess collected on aerobic swab

BA

CA (if haemophilus/neisseria suspect)

MAC

PEA

GS

Culture media for blood

Automated detection

--1 aerobic bottle culture bottle

--1 anaerobic blood culture bottle

Culture media for bone and joints

BA

CA

MAC

Thio

GS

BA

blood agar

SSA

Strep selective agar

MAC

MacConkey agar

CA

Chocolate agar

GS

gram stain

SS

salmonella-shigella agar

HE

hektoen enteric agar

CIN

Cefsulodin-irgasan-novobiocin agar

TM

thayer-martin agar

Thio

thioglycolate broth

Culture media for feces/rectal swab spec

Mod. diff/selective--> MAC, EMB

Highly diff.selective--> HE, XLD

Culture media for genital spec

TM

GS

LIM or Todd-Hewitt broth

For routine culture (rare): BA, CA, MAC, PEA, TM, GS

Explain an instance where TM agar would be used for genital spec culturing

to isolate neisseria gonorrhea

Why would a LIM or Todd-Hewitt broth be used for culturing a genital spec

it isolates for Group B strep (its an enriched selective broth)

PEA

Phenylethyl alcohol agar

What do selective agars do?

needed for recovery of specific pathogens

What does differential agar do?

needed to tell different organisms apart

Throat: potential pathogens

Group A strep (pyogenes)

Group C and G strep

Arcanobacterium haemolyticum

Eye: potential pathogens

Haemophilus influenza

Moraxella spp.

Strep pneumoniae

Staph aureus

S. pyogenes

Middle ear: potential pathogens

Staph aureus

Strep pyogenes

H. influenzae

Anaerobic bacteria

Moraxella catarrhalis

Sputum,. bronchial wash, lower respiratory spec: potential pathogens

Strep pneumoniae

Klebsiella pneumoniae (enterobacteria)

Pseudomonas aeruginosa

Staph aureus

H. influenzae

Moraxella catarrhalis

Feces: potential pathogens

Campylobacter jejuni

Campylobacter spp.

Salmonella

Shigella

E. coli

Vibrio cholerae

Yersinia

Aromonas

Listeria

Urine: potential pathogens

Enterobacteria (E. coli, klebsiella, proteus)

Enterococcus

Pseudomonas aeruginosa

Staph aureus

S. saprophyticus

Genital: potential pathogens

Neisseria gonorrhea

Heamophilus ducreyi

Group B strep

Candida albicans (yeast)

CSF: potential pathogens

Enetrobacteriae

Staph aureus

Strep pyogenes

Pseudomonas aeruginosa

Enterococcus spp.

Abscess/wound: potential pathogens

Anaerobes

Clostridium

Stap aureus

Strep pyogenes

Bacteroides spp

Enterobacteriacaea

Enterococcus

Vibrio vulnificus

Blood: potential pathogens

Viridians strep

Enterococcus

Group ABD strep

Pseudomonas spp

Neisseria spp

Anaerobes

Candida

Listeria

Bone/joint: potential pathogens

Staph aureus

Strep spp

Enterococcus spp

Pseudomonas aeruginosa

Enterobacteriacaea

Anaerobes

Neisseria gonorrhoeae