Pure Culture

inoculation plates

Inverting ________ during incubation prevents condensation from developing, which might disturb the culture by spreading the bacterial colonies and making precise counting impossible, as well as reducing contamination concerns from airborne particles landing on the bacteria.

Streak plates

________ form when microbe colonies are transplanted from one agar media to another and there are no suitable medium or growth conditions for them to flourish in.

bacterial colonies

If the plates are not flipped during incubation, condensation may occur, disrupting the culture by spreading the ________ and making precise counting impossible.

availability of oxygen

The ________ depends on whether the bacteria is an obligatory aerobe, which requires a high concentration of oxygen to thrive, or a microaerophile, which requires little to no oxygen to flourish.

streak plate

Because dead bacteria usually look the same as living bacteria, you can not presume that cells on an agar surface or in broth are alive during transfer, and hence no growth will be present on the ________.