Nissl Staining and Histology Techniques Lab

Nissl stain

Method to study neural tissue morphology.

Cresyl violet acetate

Dye used in Nissl staining.

Toluidine blue

Alternative dye for Nissl staining.

Franz Nissl

Developed Nissl staining technique.

Nucleus staining

Targets genetic material in neurons.

Negatively charged molecules

Includes DNA and RNA in staining.

White matter

Does not uptake Nissl stain.

Golgi stain

Silver staining technique for neural visualization.

Dendritic branching

Visible with Golgi stain, not Nissl.

Camillo Golgi

Introduced Golgi staining method.

Myelin stain

Stains myelin sheaths in neural tissue.

Luxol-fast blue

Dye used for myelin staining.

Cerebral white matter

Targeted by myelin stain.

Histology preparation

Process of preparing tissue samples.

Fixation

Preserves tissue using fixatives like formaldehyde.

Dehydration

Removes water from fixed tissue samples.

Clearing

Uses organic solvents to render samples transparent.

Staining

Dyeing tissue for visibility under microscopy.

Mounting

Placing tissue on slides for microscopy.

Mouse brain tissue

Subject of the Nissl staining lab.

Histoclear

Organic solvent used in clearing process.

Ethanol baths

Used in dehydration and rehydration steps.

Acrytol mounting media

Used for coverslipping stained slides.