Bacterial Identification Flowcharts: Gram-Positive Cocci and Gram-Negative Bacilli

Fill-in-the-blank practice flashcards covering biochemical identification pathways and characteristics for Gram-positive cocci and Gram-negative bacilli from laboratory flowcharts.

In the Gram-positive cocci flowchart, organisms that test catalase-negative are identified as __________.

Streptococci

In the Gram-positive cocci flowchart, organisms that test catalase-positive belong to the genera __________.

Staphylococci and Micrococcus

Among the catalase-negative Streptococci, alpha-hemolysis is characterized by a __________ appearance on blood agar.

green

Among the catalase-negative Streptococci, beta-hemolysis is characterized by a __________ appearance around colonies.

clear

A catalase-negative Streptococcus that produces no hemolysis (no change) and grows on Bile Esculin is __________.

Enterococcus faecalis

An alpha-hemolytic Streptococcus that is sensitive to "P" is identified as __________.

S. pneumoniae

An alpha-hemolytic Streptococcus that is not sensitive to "P" is identified as __________.

S. viridans

Beta-hemolytic Streptococci are differentiated by testing for sensitivity to __________.

Bacitracin "A"

The beta-hemolytic Streptococcus sensitive to Bacitracin "A" is __________ (Group A Beta-hemolytic Strep).

S. pyogenes

The beta-hemolytic Streptococcus not sensitive to Bacitracin "A" is __________ (Group B Beta-hemolytic Strep).

S. agalactiae

Catalase-positive Gram-positive cocci are initially differentiated into two groups using the __________ test.

coagulase

A catalase-positive, coagulase-positive coccus that is beta-hemolytic and ferments mannitol is __________.

S. aureus

Coagulase-negative Gram-positive cocci that produce yellow colonies are identified as __________.

Micrococcus (Micrococcus luteus)

Coagulase-negative Gram-positive cocci that do not form yellow colonies, are non-hemolytic, and do not ferment mannitol are identified as __________.

S. epidermidis

In the Gram-negative bacilli flowchart, the initial carbohydrate fermentation test used to group the bacteria is __________.

lactose

The Gram-negative bacillus characterized as a lactose fermenter, indole-positive, and urea-negative is __________.

E. coli

Among the lactose non-fermenting Gram-negative bacilli, an organism that gives a strong positive urea reaction is __________.

P. mirabilis

In the Gram-negative bacilli identification scheme, Proteus mirabilis tests __________ for indole.

negative

A lactose non-fermenting bacillus that is urea negative (or weak positive), beta-hemolytic, indole negative, and produces a blue-green pigment on TSA with a grape-like odor is __________.

P. aeruginosa