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Maxam Gilbert
Chemical degradation - base specific break
Sanger
Chain termination
Dideoxynucleotide
Lack 3’ OH
Prevent PO bond
1 Tube - Sanger
Template DNA
Single primer
DNA pol
4dNTPs
1 ddNTP
Gel - Traditional Sanger
Polyacrylamide
Cycle Sequencing
Fluorescent labels
Gel - Cycle
Capillary + slab gel
Cycle Sequencing - How Labels Detected
Laser
Single Molecule Sequencing
Nanopore
Next Generation Sequencing
Millions of fragments simultaneously
Next Gen Sequencing - Examples
454
SOLiD
Illumina
Ion torrent