Chymotrypsin Mechanism/Plots/Common Carbohydrates

What happens in step 1?

Step 1: Substrate binding - The substrate fits into the enzyme’s active site, positioning the peptide bond next to the catalytic serine

What happens in Step 2?

Step 2: Nucleophilic attack — The serine’s O⁻ attacks the carbonyl carbon of the peptide bond, forming a tetrahedral intermediate.

What happens in Step 3?

Step 3: Substrate cleavage — The peptide bond breaks, releasing the first product (the amine group), while the acyl-enzyme intermediate remains attached.

What happens in Step 4?

Step 4: Water comes in — A water molecule enters the active site to replace the first product.

What happens in Step 5?

Step 5: Water attacks — Water, activated by histidine, attacks the carbonyl carbon of the acyl-enzyme intermediate, forming another tetrahedral intermediate.

What happens in Step 6?

Step 6: Break-off from the enzyme — The bond between the enzyme and the substrate breaks, regenerating the serine side chain.

What happens in Step 7?

Step 7: Product dissociates — The second product (carboxylic acid) is released, and the enzyme returns to its original state.

What is the full chymotrypsin mechanism?

• Step 1: Substrate binding - The substrate fits into the enzyme’s active site, positioning the peptide bond next to the catalytic serine

• Step 2: Nucleophilic attack — The serine’s O⁻ attacks the carbonyl carbon of the peptide bond, forming a tetrahedral intermediate.

• Step 3: Substrate cleavage — The peptide bond breaks, releasing the first product (the amine group), while the acyl-enzyme intermediate remains attached.

• Step 4: Water comes in — A water molecule enters the active site to replace the first product.

• Step 5: Water attacks — Water, activated by histidine, attacks the carbonyl carbon of the acyl-enzyme intermediate, forming another tetrahedral intermediate.

• Step 6: Break-off from the enzyme — The bond between the enzyme and the substrate breaks, regenerating the serine side chain.

• Step 7: Product dissociates — The second product (carboxylic acid) is released, and the enzyme returns to its original state.

What plot is this?
a. Ping-Pong Kinetic Mechanism 
b.Competitive Inhibition 
c. Sequential Kinetic Mechanism 
d. Mixed Inhibition 
e. Uncompetitive Inhibition

c. Sequential Kinetic Mechanism 

What plot is this?
a. Ping-Pong Kinetic Mechanism 
b.Competitive Inhibition 
c. Sequential Kinetic Mechanism 
d. Mixed Inhibition 
e. Uncompetitive Inhibition 

a. Ping-Pong Kinetic Mechanism 

What plot is this?
a. Ping-Pong Kinetic Mechanism 
b.Competitive Inhibition 
c. Sequential Kinetic Mechanism 
d. Mixed Inhibition 
e. Uncompetitive Inhibition

b.Competitive Inhibition 

What plot is this?
a. Ping-Pong Kinetic Mechanism 
b.Competitive Inhibition 
c. Sequential Kinetic Mechanism 
d. Mixed Inhibition 
e. Uncompetitive Inhibition

e. Uncompetitive Inhibition

What plot is this?
a. Ping-Pong Kinetic Mechanism 
b.Competitive Inhibition 
c. Sequential Kinetic Mechanism 
d. Mixed Inhibition 
e. Uncompetitive Inhibition

d. Mixed Inhibition 

Is this:
a. Ribose
b. Glucose
c. Fructose
d. Galactose
e. Mannose

Ribose

Is this:
a. Ribose
b. Glucose
c. Fructose
d. Galactose
e. Mannose

Glucose

Is this:
a. Ribose
b. Glucose
c. Fructose
d. Galactose
e. Mannose

Fructose 

Is this:
a. Ribose
b. Glucose
c. Fructose
d. Galactose
e. Mannose

Galactose

Is this:
a. Ribose
b. Glucose
c. Fructose
d. Galactose
e. Mannose

Mannose