[MP] MICRO FINALS LAB MOVING EXAM

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23 Terms

1
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A. Plate media

B. test tube media

C. Nutrient agar

D. Nutrient broth


  1. Dispensed before autoclave

  2. Dispensed after autoclave

  3. Poured in plate

  4. Poured in test tube


  1. B

  2. A

  3. C

  4. C OR D


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A. Solid

B. Semi-solid

C. Liquid

D. Complex

E. Chemically defined


  1. No agar; or broth

  2. 0.5-0.75% agar

  3. Exact composition known

  4. Incompletely known composition


  1. C

  2. B

  3. E

  4. D


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A. Solid

B. Semi-solid

C. Liquid

D. Complex

E. Chemically defined


  1. No agar; or broth

  2. 0.5-0.75% agar

  3. Exact composition known

  4. Incompletely known composition


  1. C

  2. B

  3. E

  4. D


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A. Nutrient Agar/Broth

B. Blood Agar

C. MacConkey Agar

D. Chocolate Agar

E. Bile Esculin Agar

F. Thioglycollate Broth


13. General-purpose

  1. Selective and differential

  2. Enriched and differential

  3. Differential

  4. Supports anaerobic growth

  5. Enriched


  1. A

  2. C

  3. B

  4. E

  5. F

  6. D



5
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A. Stab method

B. streak zigzag method

C. Stab and streak zigzag method

D. Suspend and shake method

  1. Agar butt/slant

  2. Agar slant

  3. Nutrient broth

  4. Agar deep/butt



  1. C

  2. B

  3. D

  4. A


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A. Stab method

B. streak zigzag method

C. Stab and streak zigzag method

D. Suspend and shake method

  1. Agar butt/slant

  2. Agar slant

  3. Nutrient broth

  4. Agar deep/butt



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A. Agar Plate

B. Agar Slant

C. Agar Deep/Butt

D. Culture Broth


  1. short term storage, maintenance, and transport

  2. determine oxygen requirements

  3. isolation of pure colonies

  4. grow large numbers of bacteria


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GIVE THE USE OF THE FOLLOWING:

  1. Nutrient Agar Plate

  2. Mueller-Hinton Agar Plate


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A. Uninterrupted overlapping (UIO)

B. Uninterrupted non-overlapping (UINO)

C. Interrupted overlapping (IO)

D. Interrupted non-overlapping (INO)

E. Streak plate method

F. Spread plate method

G. Pour plate method


  1. sample is introduced to molten agar

  2. continuous, crossing-over

  3. flame-sterilize between, crossing-over

  4. continuous, separate sections

  5. flame-sterilize between, separate sections

  6. sample is introduced to solidified agar

  7. sample is inoculated to isolate colonies


  1. G

  2. A

  3. C

  4. B

  5. D

  6. F

  7. E


10
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39-40. Give two factors affecting bacterial growth and distribution

41-42. Give two applications in clinical microbiology.

39-40. Nutrient Availability, Incubation Temp, Oxygen Availability, Inoculum Density

41-42. Isolation of Microorganisms, Quantitative Enumeration, Culture Maintenance & Cultivation, and Antimicriobial Susceptibility Testing Preparation


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A. Staphylococcus

B. Serratia

C. Micrococcus

D. Chromobacterium


  1. yellow

  2. violet

  3. red

  4. gold


  1. C

  2. D

  3. B

  4. A


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<p>Give the following forms of the agar slant: (+1 pt if u enumerated even the others)</p>

Give the following forms of the agar slant: (+1 pt if u enumerated even the others)

  1. Beaded

  2. Rhizoid (rootlike)

  3. Spreading

BONUS (frm left to right): Arborescent, Echinulate, Filiform

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A. Bacillus subtilis

B. Staphylococcus aureus


Match the bacteria based on their characteristics in agar slant:

  1. diffuse

  2. moderate growth

  3. golden-yellow

  4. filiform

  5. creamy white


  1. A

  2. B

  3. B

  4. B

  5. A


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A. Bacillus subtilis

B. Staphylococcus aureus


Match the bacteria based on their characteristics in broth culture:

  1. viscous

  2. scanty growth

  3. slightly flocculent

  4. sediment at bottom


  1. A

  2. A

  3. B

  4. A


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A. Bacillus subtilis

B. Staphylococcus aureus


Match the bacteria based on their characteristics in deep/butt:

  1. motile

  2. non-motile

  3. peritrichous flagella


  1. A

  2. B

  3. A


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<ol start="61"><li><p>A 2-5 mm colony size is _____.</p></li><li><p>A colony measuring less than 1mm is _____.</p></li><li><p>A colony shaped like in this picture is _______.</p></li><li><p>A colony that is shiny and glistening is ____ texture.</p></li><li><p>Texture of mycobacterium tuberculosis.</p></li></ol><p></p>

  1. A 2-5 mm colony size is _____.

  2. A colony measuring less than 1mm is _____.

  3. A colony shaped like in this picture is _______.

  4. A colony that is shiny and glistening is ____ texture.

  5. Texture of mycobacterium tuberculosis.


  1. Moderate

  2. Pinpoint

  3. Erose

  4. smooth

  5. wrinkled (considered rough too)


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<ol start="66"><li><p>Name the elevation based on the pic.</p></li><li><p>Cushion-shaped</p></li><li><p>Dome-shaped</p></li><li><p>Colony is pricked and comes off whole in the agar surface</p></li><li><p>Pearly sheen when reflected w/ light</p></li></ol><p></p>

  1. Name the elevation based on the pic.

  2. Cushion-shaped

  3. Dome-shaped

  4. Colony is pricked and comes off whole in the agar surface

  5. Pearly sheen when reflected w/ light


  1. Umbonate

  2. Pulvinate

  3. Convex

  4. Rubbery

  5. Opalescent


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A. Bacillus subtilis

B. Staphylococcus


Match the bacteria based on their characteristics in agar slant:

  1. Medium to large

  2. Undulate edge

  3. Flat elevation

  4. Butyrous

  5. Entire edge

  6. Rough

  7. Smooth

  8. Convex elevation

  9. Crumbly

  10. Pale beige


  1. A

  2. A

  3. A

  4. B

  5. B

  6. A

  7. B

  8. B

  9. A

  10. A



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A. Susceptible

B. Intermediate

C. Resistant


  1. Penicillin in S aureus

  2. CIP10 in B subtilis

  3. CN10 in S aureus


  1. C

  2. A

  3. B


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  1. What McFarland level is used as standard?

Define the ff:

  1. Antimicrobial agent

  2. Antibiotic

  3. Zone of inhibition


  1. 0.5

  2. kills microbes IN or OUT of the body

  3. kills or inhibits BACTERIA.

  4. ring that helps interpret antimicrobial strength of chemical agent/ antibiotic.


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  1. Chemical agent involved in betadine

  2. Chemical agent involved in liquid lysol

  3. Chemical agent involved in H2O2.

  4. Chemical agent involved in listerine.

  5. Chemical agent involved in detergent


  1. Povidine-iodine (iodine)

  2. alkyl dimethyl benzyl ammonium chloride (ADBAC) or quaternary ammonium cmpds

  3. reactive oxygen species (hydroxyl radicals)

  4. essential oils, ethanol, or benzoic acid

  5. Surfactants or chelators


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  1. Why is MHA used for both antibiotic and chemical sensitivity testing?

Define the ff:

  1. Bacteriostatic agent

  1. Bactericidal agent

  2. A good disinfectant

  3. A good antiseptic


  1. Give other ways to determine chemical agent effectiveness in bacteria.


  1. It allows disk diffusion easily than regular nutrient agar (many possible ans)

  2. used for controlling or preventing the growth of bacteria

  3. used for killing bacteria

  4. kills bacteria on non-living things while being compatible w surface, quick acting, and non toxic.

  5. Kills microbes on living tissues while being safe to use, low adverse effects, low cytotoxicity

  6. Dilution or use-dilution test


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  1. Among the chemical agents used, which has the largest zone of inhibition?

  2. Added to broth to standardize it to McFarland turbidity test.


  1. Hydrogen peroxide

  2. Normal Saline Solution (NSS)