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B. anthracis
Bamboo-shaped and Medusa Head; non-motile.
Listeria monocytogenes
Umbrella motility; cold (4°C); actin rockets.
C. diphtheriae
Club-shaped; gray pseudomembrane; bull neck.
Lactobacillus
Filamentous; catalase negative; vaginal flora.
H. influenzae
Satellite phenomenon; needs X and V factors.
H. ducreyi
School of fish appearance; painful ulcer; needs X only.
B. pertussis
Mercury drop colonies; associated with whooping cough.
Brucella
Causes undulant fever; associated with livestock; urease positive.
E. coli
Metallic green sheen on EMB; indole positive.
K. pneumoniae
Mucoid/snotty; non-motile; currant jelly sputum.
Serratia marcescens
Red pigment at room temperature; DNAse positive.
Proteus mirabilis
Swarming rings; burnt chocolate smell; urea positive.
Salmonella Typhi
H2S positive; motile; associated with gallbladder.
Shigella
H2S negative; non-motile; causes bloody stool.
Yersinia pestis
Safety pin stain; associated with fleas; fried egg colony.
P. aeruginosa
Blue-green pigment; grapes/taco smell; CF/burns patients.
V. cholerae
Yellow on TCBS; rice-water stool; string test positive.
V. parahaemolyticus
Green on TCBS; halophilic; needs salt.
V. vulnificus
Hemorrhagic bullae; associated with liver disease.
C. jejuni
Grows at 42°C; seagull wings appearance; associated with poultry.
H. pylori
Strong urease positive; associated with gastric ulcers.
TCBS culture media
Used for Vibrio identification (Yellow=Cholera, Green=others).
Bordet-Gengou
Culture media for Bordetella pertussis.
Chocolate Agar
Media for Neisseria and Haemophilus growth.
BCSA
Culture media for Burkholderia (dirt smell).
EMB agar
Used for E. coli identification (green sheen).
Fastidious bacteria
Bacteria that require complex growth factors.
Seagull wings appearance
Characteristic morphology of Campylobacter jejuni.
Indole test
Test for the ability to convert tryptophan to indole.
Urease test
Tests for the presence of urease enzyme that hydrolyzes urea.
Catalase test
Differentiates between staphylococci (catalase positive) and streptococci (catalase negative).
H2S production
Production of hydrogen sulfide, a characteristic of some enteric bacteria.
Actin rockets
Mechanism used by Listeria monocytogenes to move within host cells.