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What does metabolism include
The physical role of nutrients and intermediary metabolism.
What is energy metabolism
The overall use of chemical energy, including oxidation of food to produce ATP.
What units are used to measure energy
Calories/kcal, joules, and watts.
What is the difference between potential and kinetic energy
Potential energy is stored chemical energy. Kinetic energy is energy associated with motion or heat.
What happens to food energy during digestion and absorption
Energy is transferred into ATP and transformed into heat because the process is inefficient.
What are the major uses of energy in animals
Maintenance, biosynthesis, and physical work.
Why do maintenance, biosynthesis, and physical work produce heat
These processes are inefficient, so some energy is transformed into heat.
How does high metabolism contribute to endothermy
Heat produced by high metabolism maintains high body temperatures in birds and mammals.
How can diet trials measure energy expenditure
Provide diets with different energy levels, monitor body mass gain/loss, and determine intake required for 0% mass change.
How do digestibility studies measure energy expenditure
Measure the energy value of all food consumed and all material excreted.
What assumption is required for energy-focused digestibility studies
No change in body mass or body composition.
What is direct calorimetry
Measuring total heat production to determine energy expenditure.
What is the advantage and disadvantage of direct calorimetry
Most accurate in principle but difficult in practice.
What is indirect calorimetry
Using gas exchange to approximate heat production.
Why can O₂ consumption estimate heat production
Heat produced per volume of O₂ consumed is nearly constant regardless of substrate.
What differs among carbohydrates, lipids, and proteins during oxidation?
The amount of CO₂ produced.
What is respiratory quotient (RQ)?
Volume of CO₂ produced divided by volume of O₂ consumed.
What does RQ indicate?
Which metabolic substrate an animal is oxidizing.
What is the RQ of carbohydrates?
1.0.
What is the RQ of lipids?
0.71.
What is the RQ of proteins?
0.83.
What is doubly labeled water used for?
Measuring energy expenditure in the field.
How does doubly labeled water work?
The animal receives stable isotopes ²H and ¹⁸O. Hydrogen is lost as water, while oxygen is lost as water and CO₂.
How is energy expenditure calculated using doubly labeled water?
The difference in isotope loss estimates CO₂ production, and energy expenditure is calculated using RQ.