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what is the method of limits
Stimuli are presented in ordered, step-by-step sequences of either ascending (subthreshold → suprathreshold) or descending (suprathreshold → subthreshold) series until the observer's response shifts at a transition point
subthreshold
stimulus below threshold
suprathreshold
stimulus above threshold
how do we measure absolute threshold in classical psychophysical methods
the stimulus is presented in isolation as we establish what is the minimum light intensity for observer to detect the stimulus
how do we measure difference threshold in classical psychophysical methods
stimulus is presented against a background and we find the minimum difference in light intensity between the stimulus and the background so that the observer is able to detect the difference
describe descending method of limit
start with stimuli well above threshold and decrease light intensity as experiment progress until observer unable to detect stimulus
describe ascending method of limits
stimulus presented initially at low intensity below the observer’s threshold, and gradually the intensity of the stimulus is increased
why do we need several trials to calculate an average threshold for method of limit
because observer’s threshold fluctuates from time to time
what is interval of uncertainty (IU)
stimulus range over which the subject does not notice a difference between the reference and comparison stimulus
formula for IU
IU = upper limen – lower limen
formula for difference threshold for the method of limit
DL = IU / 2 = (LU - LL)/2
define upper limen
Point where judgment shifts between "Equal" and "Greater"
define lower limen
Point where judgment shifts between "Less" and "Equal"
formula for Point of Subjective Equality (PSE)
PSE = (LU+LL)/2)
define point of subjective equality (PSE)
point where the reference and comparison stimulus are perceived as equal (midpoint between LU and LL)
define point of objective equality (POE)
point where the reference and the comparison stimulus are measured as equal using an objective technique
formula for constant error
CE = POE - PSE
what is constant error
the difference between POE and PSE
what happens when we combine ascending and descending limits
the habituation and anticipation are cancelled out because the patient is not habituated in one direction of responses and they don’t anticipate a certain response
advantages of method of ascending limits
does not dirupt adaption of subject especially dark adaptation
disadvantages of method of ascending limits
patient’s anticipation
habituation
practice
fatigue
not knowing what the stimulus is before experience starts
precautions for method of ascending limits
ensure observer understands the test
test multiple times for reliability
vary the starting point to prevent habituation
example of method of ascending limits
visual fields (perimetry)
advantages of method of descending limits
patient familiarized with stimulus
obtains practice before reaches threshold
disadvantages of method of ascending limits
disrupts adaptation especially dark
patient’s anticipation
habituation
fatigue
precautions for descending method of limits
ensure observer understands the test
test multiple times for reliability
vary the starting point to prevent habituation
example of method of descending limits
snellen visual acuity
how do we determine the difference threshold using method of limits
the observer needs to make a comparison between a standard (or reference) stimulus, and a comparison (or test) stimulus
what is the adaptive method of method of limits
staircase method
how does the staircase method work
The stimulus intensity automatically reverses direction (ascending <–> descending) whenever the observer's response changes
what is the transition point in the staircase method called
reversal
how do we determine the observer’s threshold with the staircase method
using the average of the last three or four reversals
the direction of the stimulus of a staircase method depends on what
the observer’s responses
advantages of the staircase method
keeps stimulus close at threshold always and won’t disrupt observer’s adaptation
disadvantages of the staircase method
boredom
learning effect
how to prevent disadvantages in staircase method
use the double random staircase method
define double random staircase method
The two staircase stimuli are randomly presented to the observer so that they don’t know what to expect. Each set of reversals will be analyzed independently to measure their thresholds.
what do we do if it takes too long to reach the first reversal in the staircase method
use the Parameter Estimation by Sequential Testing (PEST)
define Parameter Estimation by Sequential Testing (PEST)
it uses a different approach to achieving reversals without causing observers to experience fatigue or boredom
how does PEST differ from regular staircase method
The step of decreasing or increasing the stimulus’ characteristic is not constant throughout the test, but changes depending on the observer’s response
how does PEST work
Stimulus is first presented as either subthreshold or suprathreshold
Depending on the observer’s response, the next stimulus’s value will change by a large step, eliciting the opposite response from the observer
advantages of Parameter Estimation by Sequential Testing (PEST)
Concentrates testing right around the threshold, dramatically shortening test duration and minimizing patient fatigue
example of PEST
Automated Visual Field testing (Perimetry) in glaucoma screening
golden standard of psychometric test
method of constant stimuli
define method of constant stimuli
Predefined stimuli shown in random order and the observer is asked if they see the stimulus by responding with YES or NO
why is the observer exposed to each value of stimuli multiple times during the experiment
for higher accuracy
how is the Psychometric Function or the Frequency of Seeing (FOS) Curve plotted
number of YES responses as a function of stimulus intensity
how do we determine the stimuli used in the experiment for the method of constant stimuli
we need to run preliminary experiments to establish the range within which the threshold for our observer population might be
advantages of method of constant stimuli
no habituation
no anticipation
disadvantages of method of constant stimuli
time consuming
why is the method of constant stimuli the golden standard
it yields highly accurate estimates of absolute threshold
what can FOS curve reveal even if the threshold is calculated to be within normal limits
abnormalities on the detection of a stimulus
what is the S-shaped function that reveals abnormalities on the detection of a stimulus in the FOS curve
ogive
how to measure difference threshold for the method of constant stimuli
a reference stimulus and a comparison stimulus are shown to the subject
the value of the comparison stimulus is changed from trial to trial and the subject is required to judge whether this value is higher or lower than the value of reference stimulus
what is the LL in method of constant stimuli for difference threshold
intensity value at 25% higher
what is the LU in method of constant stimuli for difference threshold
intensity value at 75% higher
x and y axis for ideal/real observer
intensity, % detected
x and y axis for x and y axis for the method of limits
presentation sequence and stimulus intensity
x and y axis for the method of constant stimuli
stimulus intensity and % YES
x and y axis for the method of constant stimuli - difference threshold
stimulus intensity and % higher
what is PSE on the method of constant stimuli for difference threshold
intensity value at 50% higher responses
in method of constant stimuli, what does a steep psychometric function mean?
small difference limen (DL) and high sensitivity. Observer responses changes decisively from not greater to greater over a very narrow range of stimulus values
in method of constant stimuli, what does a shallow psychometric function mean?
large difference limen (DL) and low sensitivity (large change in stimulus intensity is needed before the observer ‘s judgement changes
define method of adjustment
Observer adjusts the stimulus using the criterion given by the experimenter and each trial begins with a subthreshold or suprathreshold stimulus
how to measure the absolute threshold in the method of adjustment
average of multiple thresholds defined during multiple trials
advantages of the method of adjustment
natural and straightforward to observer, observer won’t lose interest if they in control of experiment
disadvantages of the method of adjustment
experimenter does not have control of experiment
takes time to complete test for inexperienced observers
observers may use cues in experimental setup to set the threshold
If use method of adjustment to measure the difference threshold for an observer, what is it called
method of average error
how to obtain method of average error
it requires a reference stimulus and a test stimulus and the patient to adjust the test stimulus until it matches the reference stimulus
what is the matching point in the method of average error
point of subjective equality (PSE)
in the method of average error, what does the experiment do after knowing the point of objective equality (POE)
they can use it to calculate the constant error for each trial
what is the difference threshold in method of adjustment
it is a range of difference thresholds instead of a single value because constant errors are normally distributed
how is POE and CE distributed
normally distributed or bell shaped
when plotting PSE for difference threshold in the method of adjustment, what is the x and y axis
constant error, frequency with which L is seen as equal to POE
what does a small deviation mean in the difference threshold plot for method of adjustment
it means small DL (difference threshold), high precision, and the observer’s matches are very consistent
define alternative forced choice (AFC)
it presents a set of stimuli and “forces” the observer to choose from these options
why is method of forced choice used
because observers have different criteria for setting their threshold and criteria for an observer change between trials from moment to moment
example of method of forced choice for optometric test
The Jackson Cross Cylinder
x and y axis for method of forced choice
intensity and % correct
starting point for 2AFC experiment
50%
threshold for 2AFC experiment
75%
how do we obtain threshold for method of forced choices
halfway between the starting point and 100%
starting point for 4AFC experiment
25%
threshold for 4AFC experiment
62.5%
Increasing the number of choices __ the probability of guessing correctly
decreases
how do we obtain the starting point for alternative forced choice
it is the % chance of the observer getting it correctly
how must stimuli be presented for alternative forced choice
either at the same time and in separate retinal locations or at the same retinal location and at different times
how to control space error for alternative forced choice
reference stimulus will be randomly presented half the time in one location, and half the time at the other location
how to control time error for alternative forced choice
reference stimulus must be presented as first in half of the trials and as second in the other half of the trials
Method of counterbalancing spatial location or temporal order
based on assumption that when the results from all the trials are combined, the effects of the time and space errors will be cancelled, providing an unbiased estimate of DL
what is Response bias
a strong preference that an observer shows toward one or another response when making a decision
what is the method to determine degree to which an observer is guessing (guessing rate)
catch trials
formula to control for YES bias
YES_corrected = (YES - G) / (1 - G) x 100%
formula to control for NO bias
YES_corrected = (YES) / (1 - G) x 100%
guesing rate formula for biases towards YES
G = false positives/all trials
guesing rate formula for biases towards NO
G = false negatives/all trials
when will the correction formulae for response bias work
only under the assumption that the guessing rate is constant throughout the range of stimuli (assume that the observer’s rate of bias does not change)
we do not need to use catch trials under which method
method of constant stimuli

ideal observer

real observer