CH 7 Acid-Fast Staining

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Last updated 10:02 PM on 10/2/26

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1

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For which organisms is Acid Fast staining done for?

Mycobacteria and Acid-fast parasites

2

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What feature in these acid fast organisms allow them to be stained?

MYCOLIC ACID

Mycolic acid + Carbolfuchsin

3

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What is the FIRST step in a Kinyoun Stain?

Carbolfuchsin for 5 MINUTES

then rinse with DI water

4

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What is the SECOND step in a Kinyoun Stain (A type of Acid-Fast)?

DECOLORIZE with ACID ALCOHOL

Rinse with DI water

5

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What is the FINAL step in a Kinyoun Stain?

Wash with METHYLENE BLUE counterstain for 1 minute

Rinse with DI