MLS ASCP blood bank high yeild

Translate D + (fisher-race) into weiner

R

Translate D = (fisher-race) into weiner

r

Translate C + (fisher-race) into weiner

1 or ‘

Translate E + (fisher-race) into weiner

2 or “

Translate ce (fisher-race) into weiner

0

Translate CE (fisher-race) into weiner

z or y

What race is e most common in

White

What race is almost always D+

Asains

Which antigen types are the most common

R1 (DCe) and r (dce)

What is the immunodominant sugars for A blood group

N-acetylgalactosamine (GaINAc)

What are the immunodominant sugars for the B blood group

D-galactose

What are the immunodominant sugars for AB blood group

N-acetylgalactosamine (GaINAc) And d-galactose

What is the immunodominant sugars for the O blood group

H antigen w/terminal L-fucose

Why do we expect to see ABO antibodies in all patients but not any blood groups

ABO antibodies are naturally occurring antibodies while most other blood group antibodies are immune antibodies that require exposure to the antigen through transfusion or pregnancy

What ABO/Rh typing result suggests a subgroup of A

Anti-a weak/mixed field

Anti-b neg

Anti-A lectin neg

A2 cells neg

B cells pos

List the ABO types by the most amount of H to least amount of H

O > A2 > B > A2B > A1 >A1B

What ABO phenotype will be seen in a person whose genotype is A, O, hh and what blood type is this called

O phenotype, bombay phenotype (Oh)

What ABO antibodies will be expected in a bombay phenotype

Anti-a anti-b anti-H

What blood can be transfused to a bombay person

Only bombay blood

What Rh phenotype will we see in a patient who is Rh null and what type of RBC can they receive

D- C- E- c- e-, only Rh-null blood

What antibody do we expect to see in all Rh null patients

Anti-Rh29

Which genes are inherited to make someone a secretor

FUT2 (Se) gene:

SeSe secretor

Sese secretor

sese nonsecretor

What reaction is expected in a specimen if the patient is a secretor or non secretor

Secretor- no agglutination

Non secretor- agglutination

^in saliva inhibition/neutralization

If mom is O and dad is AA what ABO type can their children be

All children will be type A (AO)

If mom is O and dad is AO what ABO types can their children be

50 % type A (AO) 50 % type O (OO)

If mom is JK(a+b-) and dad is JK(a-b+) what are the possible JK antigen combinations could the children inherit

All children will be JK(a+b+)

What is the use of LISS

Decreases the ionic strength of the medium, increases rate of antibody uptake, enhances IgG antibody reactions and shortens incubation time

What is the use of albumin

Decreases the zeta potential between RBCs allowing the RBCs to come closer together, enhances agglutination especially with some IgG antibodies

What is the use of PEG

Removes water from the testing environment, concentrates antibodies, strongly enhances IgG reactions and is used in antibody detection, requires thorough washing before AHG is added

What is the use of AHG

Detects RBCs coated with IgG and/or complement, bridges sensitized RBCs so agglutination can be observed

Which blood type makes anti-a,b

Group O

Which blood group antigens are enhanced by enzymes

Rh, kidd, lewis, p1, I

Which blood groups are destroyed by enzymes

Duffy (fyb fya) MNS

When should an elution be performed

After a positive DAT

What is the difference between DAT and IAT

DAT: direct detects IgG and/or complement already attached to patient RBC in vivo

IAT: indirect detects Ab in patient serum that can bind RBC antigen in vitro

What does the rosette test (fetal screen) detect

D positive fetal RBCs in a D negative mothers circulation

When do you perform a KB test

After a positive rosette test

Why is KB test performed

To quantify fetal RBCs in maternal circulation and determine the dose of RHIG

What is the cause of HDFN

Maternal IgG alloantibodies that cross the placenta and destroy fetal RBCs

What is the cause of GVHD

Donor T lymphs attacking the recipients tissue after transfusing a graft, give irradiated blood

What is platelet refractoriness

After a platelet transfusion, the patients platelet count does not increase and improve even after multiple transfusions

What is the expiration time of RBC stored in CPD

21 days

What is the expiration time of RBC stored in CPDA

35 days

What is the expiration time of RBC stored in AS-1, AS-3, AS-5

42 days

What diseases does an allogenic donor need to be tested for

HIV 1/2, HBV, HCV, HTLV I/II, syphilis, WNV, trypanosoma cruzi, babesia, CMV

What antibodies are naturally occurring

ABO, Lewis, P1, MN, Lua

What antibodies are clinically significant

ABO, Rh, Kell, Duffy, Kidd, SSU

What antibodies are warm antibodies

Rh, kell, duffy, kidd

What antibodies are cold antibodies

M, N, P1

What antibodies only react in AHG (not detected IS)

Kell, duffy, kidd

What antibody can react in phase of testing

Lewis

What antibodies are enhanced by enzyme treatment of test cells

Rh, lewis, kidd

What antibodies are not detected with enzyme treatment of test cells

M, N, duffy

What antibodies show dosage

Rh other than D, MNS, duffy, kidd

What RBCs can an O patient receive

O

What RBCs can an A patient receive

O, A

What RBCs can an B patient receive

O, B

What RBCs can an AB patient receive

O, A, B, AB

What type of plasma can a type O patient receive

O, A, B, AB

What type of plasma can a type A patient receive

A, AB

What type of plasma can a type B patient receive

B, AB

What type of plasma can a type AB patient receive

AB

What type of platelets/cryoprecipitate can a type O, A, B, or AB patient receive

O, A, B, AB

What type of whole blood can a type O patient receive

O

What type of whole blood can a type A patient receive

A

What type of whole blood can a type B patient receive

B

What type of whole blood can a type AB patient receive

AB

What type of granulocytes can a type O patient receive

O

What type of granulocytes can a type A patient receive

O, A

What type of granulocytes can a type B patient receive

O, B

What type of granulocytes can a type AB patient receive

O, A, B, AB