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What is aquaponics?
A system combining aquaculture and hydroponics where fish waste fertilizes plants and plants clean the water.
What is hydroponics?
Growing plants in nutrient-rich water without soil.
Agriculture vs aquaculture advantages
Agriculture: large food production. Aquaculture: controlled environment, efficient protein.
Agriculture vs aquaculture disadvantages
Agriculture: soil depletion, weather risks. Aquaculture: disease/water quality risks.
Role of botanist
Plant care, germination, transplanting, harvesting.
Role of zoologist
Fish care, feeding, monitoring behavior.
Role of communications/media manager
Documentation, photos, announcements, reports.
Role of data analyst
Tracks water quality data and analyzes trends.
Role of test manager
Conducts water tests and identifies issues.
Role of system manager
Monitors pumps, valves, airflow, equipment.
Clarifier purpose
Removes solid fish waste.
Biofilter purpose
Houses bacteria that convert ammonia → nitrite → nitrate.
Raft bed purpose
Area where plants grow on floating rafts.
What is titration?
A test where reagent drops are added until color changes.
Which tests require titration?
Alkalinity, hardness, CO₂, ammonia (depending on test kit).
What is germination?
Seed sprouting.
When to place germination trays under light?
When seedlings emerge and have leaves.
What does lettuce need to germinate?
Moisture, warmth, darkness.
What goes on a plant label?
Date and plant type; later add tray/location after transplant.
What is biosecurity?
Preventing contamination or disease entering the lab.
How to biosecure entering lab?
Use biosecurity mats, sanitize hands, wear PPE.
Fish in the system
Channel Catfish – Ictalurus punctatus.
Characteristics of catfish
Barbels, no scales, bottom dwellers, tolerate low oxygen.
How to tare a scale
Zero the scale with the container so only fish weight shows.
Why remove dead leaves?
Prevent rot, pests, algae, and contamination.
What is the NFT system?
Nutrient Film Technique; nursery stage for seedlings.
Definition of an acid
pH lower than 7.
Definition of a base
pH higher than 7.
Where do we store fish tank biomass data?
In the fish biomass log sheets.
How do we determine feed amount?
Percentage of fish biomass.
Units for mass
Grams (g).
Units for water quality
PPM or mg/L.
Units for volume
Liters (L).
What does PPM stand for?
Parts per million; same as mg/L.
Signs of stress in fish
Gasping, clamped fins, lethargy, not eating, erratic behavior.
When do you retest water?
When results are outside ideal range or after adjustments.
When rinse testing materials?
Before tests; rinse with fish water 3 times.
Two factors bad ammonia depends on
pH and temperature.
Nitrogen cycle steps
Ammonia → Nitrite → Nitrate.
Nitrogen bacteria types
Nitrosomonas converts ammonia; Nitrobacter converts nitrite.
Categories of lettuce
Crisphead, butterhead, romaine, loose-leaf.
Ways to harvest lettuce
Whole head, cut-and-come-again, or leaf-by-leaf.
What is dissolved oxygen?
Oxygen available in water for fish/bacteria.
What to do if DO is low?
Increase aeration, fix air stones or pumps.
What do pH, alkalinity, hardness measure?
pH: acidity. Alkalinity: buffering. Hardness: minerals.
How to remove chlorine
Aerate water or add dechlorinator.
What to do if nitrite nitrogen is high
Add chloride (salt) and perform partial water change.
What to do if ammonia nitrogen is high
Stop feeding, do partial water change, check biofilter.
Why fish need magnesium
Metabolism.
Why fish need calcium
Bone and tissue development.
Why fish need iron
Blood formation.
Why fish need sodium chloride
Reduces nitrite toxicity; lowers stress.
Why plants need magnesium
Chlorophyll.
Why plants need calcium
Cell wall strength.
Why plants need carbon dioxide
Photosynthesis.
Coupled vs decoupled systems
Coupled = one-loop. Decoupled = fish and plants in separate loops.
Lab safety rules
PPE, no eating, cautious with chemicals, clean space.
Animal ethics rules
Handle fish gently and minimize stress.
How to identify tanks
Label numbers and system maps.
How to identify decoupled beds
Labels on beds and connected plumbing lines.
Water/air flow through system
Fish tank → clarifier → biofilter → plant beds → sump → back to tank.
How to refill biosecurity mats
Add disinfectant solution until fully saturated.
Catfish fins
Dorsal, pectoral, pelvic, anal, caudal – used for movement, steering, balance.
Fish classification
Animalia → Chordata → Actinopterygii → Siluriformes.
How to compromise plant vs fish water needs
Maintain mid-range pH (~6.8–7.0), good aeration, clean water.
What do valves do?
Control direction and flow rate of water/air.
Four types of lettuce
Crisphead, butterhead, romaine, loose-leaf.
Three ways to harvest lettuce
Whole harvest, cut-and-come-again, leaf harvest.