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All living systems require a continuous input of energy. Describe how energy enters a cell, an organism, and an ecosystem
cell: ATP (made from sunlight by photosynthetic cells or macromolecules like glucose for autotrophs)
organism: using electromagnetic energy from sunlight to make their own chemical energy (autotrophs/photosynthesizers) or consuming other organisms to get chemical energy from food (autotrophs)
ecosystem: sunlight and geothermal energy (organisms make their own energy from sunlight which then powers the ecosystem)
What is the first law of thermodynamics?
law of conservation of energy
energy cannot be created or destroyed, but it can be changed from one form to another
What is the second law of thermodynamics?
law of efficiency
energy cannot be changed from one form to another without a loss of usable energy
each energy conversion loses energy to the environment increasing the entropy in the universe
What occurs if there is a significant loss of organization or loss of energy flow?
a cell or organism will no longer be able to maintain structure and continue with vital functions, leading to death
all living systems require a constant input of energy to fight against entropy and maintain order
What is a coupled reaction? How is ATP used in coupled reactions?
a coupled reaction occurs when a non-spontaneous/endergonic reaction is driven by a spontaneous/exergonic reaction to use energy in a more efficient way
ATP would be released by an exergonic reaction then used in an endergonic reaction
What is the formula for photosynthesis?
6H2O + 6CO2 + sunlight energy —> C6H12O6 + 6O2
H2O from soil
CO2 from atmosphere via leaves
What is the purpose of photosynthesis?
convert energy from the sun to usable energy in the form of glucose (carbohydrate)
What organisms did photosynthesis first evolve in and how did this lead to the development of modern photosynthetic eukaryotes?
ancient prokaryotic bacteria originally performed photosynthesis through their cell membranes, but by the endosymbiotic theory, the chloroplast was consumed and established a mutualistic relationship with another cell, leading to the photosynthesis we see today
What is the connection between photosynthesis and the earth’s atmosphere?
photosynthesis releases oxygen into the atmosphere and takes in carbon dioxide from the atmosphere
What does a chloroplast look like? Identify the location of the light dependent and light independent reactions

What is the purpose of the light dependent and light independent reactions?
light dependent: change energy from sunlight into usable energy (ATP and NADPH)
light independent: use ATP and NADPH to make glucose to be used for energy
What structures contain chlorophyll, and which absorbs energy from the sun and uses it to energize electrons?
chloroplasts, specifically photosystems I and II in the thylakoids, contain chlorophyll
photosystems I and II use sunlight to energize electrons
What occurs when electrons pass through the electron transport chain?
they release energy as they fall back down to their original energy level. this energy is then used to pump hydrogen ions into the thylakoid
What is chemiosmosis and how does it relate to the production of ATP during the light dependent reactions?
the movement of ions (such as H+ or protons) from a region of high concentration to a region of low concentration across a selectively permeable membrane
powers and provides the energy for the synthesis of ATP by ATP synthase
What is the electron donor for the light dependent reactions? Is this oxidized or reduced?
water, which is oxidized
What is the final electron acceptor for the light dependent reactions? Is this oxidized or reduced?
NADPH, which is reduced
What is photophosphorylation?
the process by which energy from sunlight drives the movement of electrons along an electron transport chain, leading to ATP synthesis
What is the overall purpose of respiration and fermentation?
break down energy from organic molecules, such as glucose, and convert it to ATP
Explain how the act of respiration/fermentation are characteristics of life
all living organisms must perform respiration/fermentation in order to live. the energy from these processes power essential cell processes and prevent breakdown/loss of order
How does the process of glycolysis provide evidence of the common ancestry of living organisms?
because it also occurs in prokaryotes, indicating it first evolved in prokaryotes, as well as occuring in fermentation
Compare and contrast the processes of cellular respiration and fermentation
Fermentation produces NAD+ and CO2 (in alcohol fermentation) as well as alcohol or lactic acid
Does not produce ATP, but the glycolysis before it produces 2 net ATP
Cellular respiration also includes glycolysis (producing 2 net ATP)
Also includes three other stages, producing more ATP as well as NADH, CO2 and FADH2
What does a mitochondria look like?

Where does each major process of cellular respiration take place?
Glycolysis: outside of cell in cytoplasm
Link/prep reaction: intermembrane space/mitochondrial matrix
Krebs cycle: mitochondrial matrix
Oxidative phosphorylation: inner mitochondrial membrane in the cristae (folds)
What is the role of glucose in cellular respiration?
Original source of chemical energy to eventually be turned into ATP
What is the role of pyruvate in cellular respiration?
Product of glycolysis; actually enters mitochondria and oxidized in the link/prep reaction
What is the role of NAD+/NADH in cellular respiration?
NAD+ accepts an electron to form NADH
NADH acts as an electron carrier that transports electrons to the ETC
What is the role of FAD+/FADH2 in cellular respiration?
FAD+ accepts electrons to form FADH2
FADH2 acts as an electron carrier that transports electrons to the ETC
What is the role of oxygen in cellular respiration?
Pulls electrons through electron transport chain to pump hydrogen ions into the intermembrane to allow for the formation of ATP by ATP synthase
What is the role of cytochrome c in cellular respiration?
Part of the ETC that passes the electrons to another protein within the chain. Thus it also helps form the chemiosmotic gradient necessary for the function of ATP synthase
What is the role of ATP Synthase in cellular respiration?
synthesize ATP using energy from chemiosmosis
What is the role of ADP/ATP in cellular respiration?
ADP is used to create ATP, which is then used to power cell processes
What are the inputs and outputs of glycolysis?
Inputs: glucose, NAD+, and ADP and Pi
Outputs: 2 pyruvate, NADH, and net 2 ATP
4 gross ATP
What are the inputs and outputs of link/prep reaction?
Inputs: pyruvate and NAD+
Outputs: Coenzyme A, NADH, and CO2
What are the inputs and outputs of Krebs cycle?
inputs: Coenzyme A, NAD+, FAD, ADP, and Pi
Outputs: ATP, CO2, NADH, and FADH2
Most CO2
What are the inputs and outputs of oxidative phosphorylation?
inputs: NADH, FADH2, oxygen, ADP and Pi
outputs: 36 ATP, NAD+ and FAD, H2O
Produces most ATP
In which stage of cellular respiration does the ETC occur?
Oxidative phosphorylation
Which membrane is the ETC embedded in for cellular respiration?
Inner membrane
What serves as the electron donor for the ETC in cellular respiration? Is this oxidation or reduction?
NADH and FADH2 in oxidation
What serves as the final electron acceptor of the ETC? Is this oxidation or reduction, and what is formed as a byproduct?
Oxygen in reduction. Water is formed as a byproduct
Where are the hydrogen ions pumped into as electrons move down the ETC? Do you expect the pH of this space to be acidic, neutral, or basic?
The intermembrane space
pH of this space will be acidic
What is a chemiosmotic gradient and how is it harnessed to power the production of ATP in cellular respiration?
Chemiosomosis is the movement of ions (such as hydrogen ions or protons) from a region of low concentration across a selectively permeable membrane. This movement powers and provides the energy for the synthesis of ATP by ATP synthase
What are the inputs and outputs of the light dependent reactions of photosynthesis?
Inputs: light, H2O, NADP+, ADP and Pi
Outputs: O2 (to atmosphere), H+, NADPH, ATP
What are the inputs and outputs of the Calvin cycle?
Inputs: ATP and NADPH (from LD reactions), CO2
Outputs: glucose, ADP, NADPH+ and H+ (back to thylakoid/light dependent reactions)
ATP hydrolyzed