Clinical Chemistry 2 - Laboratory - 3 - Alanine Aminotransferase - Complete

Alanine aminotransferase

ALT meaning

2.6.1.2

ALT enzyme commission number

Serum glutamic pyruvic transaminase

ALT old name

Catalyze the amino group between alanine and -ketoglutarate

ALT primary purpose

24 hours

ALT half-life

Half-life

Time it takes for a substance to be at 50% concentration in the body

Pyridoxal phosphate

ALT coenzyme

Vitamin B6

Pyridoxal phosphate common name

Liver

ALT highest concentration in these organs

Hepatocellular disorders, Hepatic parenchymal disease, Obstructive jaundice, Reye's syndrome, Heart failure or AMI, Infectious mononucleosis, Muscular dystrophy

ALT-increased conditions

Pancreas, Heart, Skeletal muscle, Red blood cells, Stomach, Gut

ALT-low-concentration organs

De Ritis Ratio

Medical term for the evaluation of liver disease

1-2

De Ritis Ratio if:

Non-viral origin

1-2

De Ritis Ratio if:

Cirrhosis

>2

De Ritis Ratio if:

Alcoholic hepatitis and Hepatocellular CA

<1

De Ritis Ratio if:

Viral origin

<1

De Ritis Ratio if:

Viral hepatitis

No

Yes/No

Will ALT increase after alcohol consumption if liver is damaged?

Karmen method

ALT method used

Coupled enzymatic reaction

ALT method used principle

L-alanine

ALT method used substrate

2-Oxoglutarate

L-Alanine + ? --(ALT)--> ...

Pyruvate + L-Glutamate

ALT reaction products

Pyridoxal phosphate

This is bound to the apoenzyme and serves as a true prosthetic group

Pyridoxal phosphate

It accepts amino-group from the first substrate alanine

Lactate dehydrogenase

2nd reaction

Pyruvate + NADH+H --(What enzyme?)--> Lactate + NAD+

NAD+

What is measured in ALT determination?

340 nm

ALT absorbance

200 uL

ALT sample volume in uL

2000 uL

ALT reagent volume in uL

60 seconds

ALT lag time

60 seconds

ALT kinetic interval

4

ALT number of readings

1745

ALT kinetic factor

37°C

ALT reaction temperature

Water

ALT blank with?

U/L

ALT conventional unit

uKat/L

ALT international unit

Bilirubin up to 30 mg/dL, Triglycerides up to 2000 mg/dL, Hemoglobin up to 2.5 g/dL

ALT interferences

Falsely increased

ALT result if Bilirubin >30 mg/dL

Falsely decreased

ALT result if Triglycerides >2000 mg/dL

Falsely increased

ALT result if Hemoglobin >2.5 g/dL

<10%

ALT activity lost within 3 days if stored at 2-8°C

<7%

ALT activity lost within 3 days if stored at 15-25°C

3 months

ALT stability at -20°C

Unhemolytic serum

ALT preferred specimen

EDTA or Heparinized plasma

ALT other accepted specimen

4.4-360 U/L

ALT measuring range

Up to 45 U/L

ALT expected value for men

Up to 34 U/L

ALT expected value for women