Microbiology Resp Lab Flashcards, Yosyraa

Streptococci in culture (Gram’s stain)

Streptococci in culture (Gram’s stain)

Streptococci in pus (Gram’s stain)

Streptococci in pus (Gram’s stain)

Pneumococci in tissue (Gram’s stain)

Pneumococci in pus (Gram’s stain)

Staphylococci in culture

Staphylococci in pus

Corynebacterium diphtheriae (Gram’s stain)

Corynebacterium diphtheriae (Methylene blue stain)

Vincent’s angina

Mycobacterium tuberculosis
(Acid fast bacilli) / (Z-N stain)

Mycobacterium tuberculosis
(Acid fast bacilli) / (Z-N stain)

Bacillus anthracis
(Gram’s stain)

Bacillus anthracis
(Gram’s stain)

Bacillus anthracis
(Spore stain)

Bacillus anthracis, McFaydean Reaction
(Polychrome methylene blue stain: blue bacilli surrounded by purple/pink capsule)

Gram negative bacilli
(Gram’s stain)

Gram negative bacilli
(Gram’s stain)

Klebsiella in tissue
(Gram’s stain)

Klebsiella in tissue
(Gram’s stain)

Candida
(Gram’s stain)

Germ Tube Test
(for differentiation of Candida albicans from other Candida spp.)
Candida albicans forms germ tubes in serum

Nutrient agar

Staphylococcus aureus on nutrient agar
(showing golden yellow endopigment)

Pseudomonas aeruginosa on Nutrient agar OR Pseudomonas exopigment on nutrient agar

Pseudomonas aeruginosa on Nutrient agar OR Pseudomonas exopigment on nutrient agar

Pseudomonas aeruginosa on Nutrient agar OR Pseudomonas exopigment on nutrient agar

Proteus on nutrient agar
(showing swarming growth)

MacConkey’s agar

MacConkey’s agar

Pink= Lactose Fermenter
Yellow= Non-lactose Fermenter

Lactose fermenter on MacConkey’s agar

Lactose fermenter on MacConkey’s agar

Lactose non-fermenter on MacConkey’s agar

Mannitol salt agar (MSA)

Mannitol salt agar (MSA)

Chocolate agar (for Haemophilus)

Blood agar

Types of haemolysis on blood agar
top left = alpha
top right = beta
lower = gamma

Alpha-haemolysis on blood agar

Beta-haemolysis on blood agar

Beta-haemolysis on blood agar

Non-haemolysis on blood agar

Non-haemolysis on blood agar

Bacitracin test (Bacitracin-sensitive organism: S. pyogenes)

Bacitracin test (Bacitracin-sensitive organism: S. pyogenes)

Optochin test
(Optochin-sensitive organism: Pneumococci)

Optochin sensitivity test

Optochin disc:
Optochin-resistant organism (viridans streptococci) (left)
Optochin-sensitive organism (pneumococci) (right)

Loffler’s serum for C. diphtheriae

Elek’s Test
Toxigenic C. diphtheriae

Elek’s Test
Non-Toxigenic C. diphtheriae

Corynebacterium diphtheriae on Blood Tellurite agar

Haemophilus influenzae on blood agar showing satellitism
(growth around Staphylococcus aureus culture)

Haemophilus influenzae on blood agar showing satellitism
(growth around Staphylococcus aureus culture)

Lowenstein Jensen (L.J.) medium
for M. tuberculosis

Lowenstein Jensen (L.J.) medium
for M. tuberculosis

Lowenstein Jensen (L.J.) medium
for M. tuberculosis

Mycobacterium Growth Indicator Tube (MGIT):
Positive fluorescence

Mycobacterium Growth Indicator Tube (MGIT):
Positive fluorescence (left arrow)
Negative florescence (right arrow)

Mycobacterium Growth Indicator Tube (MGIT):
Negative florescence

Sabouraud Dextrose agar (For Fungal culture)

Candida on Sabouraud’s dextrose agar (SDA)
colonies appear creamy white, pasty with yeasty odour

Antibiotic sensitivity test: Disc diffusion method

Antibiotic sensitivity test: Disc diffusion method

Antibiotic sensitivity test: Disc diffusion method

Biochemical reaction:
Catalase Test

Upper negative
Lower positive

organism:

staphylococci In catalase postive

streptococci in catalase negative

Biochemical reaction:
Catalase Test

left negative
right positive

organism: staphylococci

Biochemical reaction:
Coagulase Test

up coagulase positive - Staphylococcus aureus
up coagulase negative - Coagulase negative staphylococci, e.g., S. epidermidis

Biochemical reaction:
Oxidase Test

left oxidase negative
right oxidase positive

organism: pseudomonas

Biochemical reaction:
Oxidase Test

left oxidase positive
right oxidase negative

organism: pseudomonas

Biochemical reaction:
Oxidase Test

left oxidase negative
right oxidase positive

organism: pseudomonas

Biochemical reaction:
Indole Test

left Indole negative
right Indole positive

Biochemical reaction:
Citrate Test

left citrate positive
right citrate negative

Biochemical reaction:
Triple Sugar Iron (TSI)

left = TSI of carbohydrate fermenter
(e.g., E. coli, Klebsiella)

right = TSI of carbohydrate non-fermenter
(e.g., Pseudomonas aeruginosa)

Biochemical reaction:
Urease test

left = negative urea test
right = positive urea test

organism = proteus