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The light microscope
A device that uses light to visualize objects which can not
be seen with naked eye
simple microscope
has one lens
compound microscope
has a series of lens
scanning lens power
4x
largest field of view
smallest image
Low power lens power
10x'
medium image
medium field of view
High power lens power
40x
smallest field of view
largest image
Immersion oil lens power
100x
Ocular lens power
10x
parfocal lenses
Require little or no fine adjustment when moving from one to
the other objective lens
resolution of human eye
0.14 mm (140 mm) (mm = micrometer)
resolution of light microscope
0.0002 mm (0.2 mm) (mm = micrometer)
field of view
Area of the object visible through the microscope.
Depends on the objective lens used.
To measure, use ruler or stage micrometer
working distance
Distance front lens of objective and surface of cover glass, when object is in sharp focus.
Changes with magnification