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Bacteria produce the same protein products regardless of their environment.
False
The operon controls gene regulation in
prokaryotes
Promoters, operators, and structural genes are the components of a(n) __________.
operon
Which operon structure codes for the production of a repressor?
regulator gene
In prokaryotes, RNA polymerase binds to a short sequence of DNA called the
promoter
An increase in the environmental temperature would most likely cause a bacterium to
shift the expression of its proteins or enzymes
Operons are generally involved with
prokaryotic gene expression
Active repressors bind to operators which are sequences of
DNA
Which operon structure signals the start of transcription?
Promoter
Which operon structure codes for the enzymes involved in metabolic pathways of the operon?
Structural gene
The genes that code for a repressor protein in prokaryotes is known as a __________ gene.
regulator
Select the events that take place as a result of the trp operon when tryptophan is not present?
inactive repressor cannot bind operator
transcription of the trp operon takes place
T/F: RNA polymerase is able to bind to this structural gene's promoter.
True
The repressor can bind to an operator when a(n) __________ binds to it.
corepressor, co-repressor, or tryptophan
What type of pathway are repressible operons generally involved with?
anabolic pathways
What is the role of the operator?
Controls transcription of structural genes
RNA polymerase can bind to the promoter in the lac operon when
the repressor does not bind to the operator
lactose binds to the repressor
Genes that code for enzymes and proteins involved in the metabolic pathway of the operon are known as
structural genes
An inducible operon is activated by a(n)
inducer
What is the sequence of events that take place in the trp operon when tryptophan is present? Begin with the first event at the top of the list.
1. trytophan binds to the repressor
2. the repressor becomes activated
3. the repressor binds to the operator
4. transcription of the trp operon is halted
Since the CAP proteins, when active, promote the activity of the lac operon, they are said to exhibit __________ control over transcription.
positive
T/F: The trp operon is an example of a repressible operon.
True
What determines why a skin cell expresses differently than a hair cell?
The genes expressed in each cell
Repressible operons usually produce proteins that are
used often within the cell
Place these events in the correct sequence as they would occur during the functioning of the lac operon. Begin with the first event at the top of the list.
1. lactose binds to repressor
2. repressor unbinds from operator
3. RNA polymerase binds to the promoter
4. enzymes for lactose metabolism are synthesized
Which group of organisms will have their genes regulated?
Both Prokaryotes and Eukaryotes
The presence of an inducer activates a(n) __________ operon.
inducible
Of the 5 levels of eukaryotic gene expression, 3 occur in the __________ and 2 occur in the __________.
nucleus; cytoplasm
In bacteria, when glucose is present, the lac operon functions (less/more) __________; when glucose is absent, the lac operon functions (less/more) __________.
less; more
What are the levels of eukaryotic gene expression control?
Posttranscriptional control
Posttranslational control
Transcriptional control
Translational control
Chromatin structure
Even though the DNA is the same in all of the cells of a multicellular organism, cells will assume different features because they express different __________.
genes
Chromatin structure helps control gene expression by
allowing access to the DNA when it is not compacted
Pathways that produce proteins needed by the cell often contain __________ operons.
repressible
The highly compacted chromatin that is not accessible for transcription is called __________.
heterochromatin
T/F: Gene regulation occurs in both prokaryotic cells and eukaryotic cells.
True
Gene transcription is reduced on heterochromatin because it is inaccessible to
RNA polymerase
Posttranslational controls occurs in the __________ of eukaryotic cells.
cytoplasm
Which level of eukaryotic gene control is the most critical?
Transcriptional control
The first step of transcriptional control requires the presence of __________.
DNA
T/F: RNA polymerase would NOT be present in condensed eukaryotic chromatin.
True
What proteins are required for the initiation of transcription by RNA polymerase in eukaryotes?
Transcription activator
Transcription factors
Heterochromatin will have
more inactive genes than euchromatin
What structure will the transcription factors bind to?
promoter
Heterochromatin is not transcribed because
it is inaccessible to RNA polymerase
What are some of the activities of posttranscriptional control mechanisms?
control of mRNA nuclear export speed
alternative mRNA splicing
Which level of genetic control would override the others?
transcriptional
Posttranscriptional control takes place in the cell __________.
nucleus
What substance is required in eukaryotes that experience genetic control?
DNA
Eukaryotic cells have translational control occur in the
cytoplasm
What is the role of the transcription factors?
Assist in the binding of RNA polymerase to the promoter
When the activators, enhancers, and mediators come in contact with the transcription factors, the DNA forms a shape called a
loop
What can posttranslational control allow a cell to do?
Influence gene expression
Alternative mRNA splicing takes place at which level of eukaryotic gene control?
posttranscriptional control
Regulation that controls the way mRNA transcripts are processed in the nucleus are called __________ control.
posttranscriptional
The level of eukaryotic control of gene expression that involves the control of the mRNA transcript in the cytoplasm of the cell is called __________ control.
translational; translation
The level of eukaryotic control of gene expression that involves how a protein product is handled in a cell is called
posttranslational control
Where does translational control begin?
When mRNA reaches the cytoplasm
T/F: Posttranslational control is the last chance of influencing gene expression.
True
What is the most direct consequence of a gene mutation?
The organism's DNA is changed.
Mutations that occur in sex cells which can be passed to the next generation are known as __________ -line mutations.
germ
Mutations that occur in body cells and are not passed to the next generation are called __________ mutations.
somatic
A mutation that happens for no apparent reason (bad luck) is considered a
spontaneous mutation
Environmental influences, like toxic chemicals or radiation, are said to cause __________ mutations.
induced
A certain gene excerpt has the sequence AAGGGTAACGA, then changes to CCGGGTAACGA. What just happened?
A gene mutation
T/F: Spontaneous mutations resulting from errors in DNA replication are rare because DNA polymerase proofreads newly synthesized DNA.
True
What is true about germ-line mutations?
They can be passed down to the next generation
They occur in the sex cells
Which protein complex assists with DNA replication and also has the ability to repair spontaneous mutations?
DNA polymerase
Which group of cells experience mutations that can lead to cancer?
somatic cells
Identify the potential mutagen(s).
Radiation
Organic chemicals
Gene mutations that occur during normal biological processes such as movement of transposons or DNA replication are called __________ mutations.
spontaneous
A carcinogen is a type of mutagen that leads to the development of __________.
cancer
What are some of the factors that can lead to an induced mutation?
exposure to toxins
exposure to radiation
Which structure will remove thymine dimers from the DNA strand so the strand can be repaired?
DNA repair enzymes
What are some of the factors that can cause spontaneous mutations?
Transposon movement
DNA replication
Chemical change of a base
What tasks will DNA polymerase do?
Proofreads the new DNA strand for errors
Corrects errors in the DNA strand
Environmental factors such as radiation and organic chemicals can affect the nucleotide base composition of DNA and are therefore referred to as __________.
mutagens; carcinogens
Mutagens that lead to the development of cancer are called __________.
carcinogens
When one base in a DNA sequence is altered, it is known as a __________ mutation.
point
Enzymes that restore the original base sequence to altered DNA strands are known as
DNA repair enzymes
A type of point mutation where one nucleotide is replaced with another, incorrect nucleotide, is called a base __________.
substitution
What type of mutation in the DNA sequence can lead to sickle cell disease?
base substitution
What protein complex assists with DNA replication and also has the ability to repair spontaneous mutations?
DNA polymerase
The insertion of one or more bases that results in a change to the mRNA codon-reading structure is called a __________ mutation.
frameshift
Frameshift mutations are usually __________ harmful than/as point mutations.
more
The DNA sequence AAGTCTCAGGTC undergoes a point mutation. Which of the following could be the new sequence of DNA?
AAGTCTCTGGTC
If a DNA sequence that is supposed to be AAA gets changed to ATA, what specific type of mutation has occurred?
Base substitution
What are possible consequences of a mutated DNA gene?
different phenotype
different genotype
different mRNA transcript
different protein product
The DNA sequence AAGTCTCAGGTC undergoes a frameshift mutation. What could be the new sequence of DNA?
AAGTCTCCAGGTC
Which type of mutation is likely to have the most dramatic effect on an organism or cell?
Frameshift mutation
A mutation that results in one base in the DNA being replaced by a different base is called a
point mutation