Protein Purification

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Last updated 5:55 PM on 10/14/26
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4 Terms

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Size Exclusion

Column that purifies by size. Large elutes first whereas small elutes later

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Cation Exchange

Negatively charged resin attracts positively charged protein. Negative protein elute first

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Anion Exchange

Positively charged resin and negatively charged protein

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Affinity chromatography

Resin is linked to a ligand and binds to protein of interest