Unit 10: Protein and Amino Acid tests

What is the substrate for lysine broth?

lysine

What is the substrate for Phenylalanine slant?

phenylalanine

what is substrate for Urea

urea

What is substrate for Sulfur, Indole, Motility

Cysteine theosulfate, tryptophan, deep

What is enzyme for Lysine

lysine decarboxylase

What is enzymes for Phenylalanine?

phenylalanine deaminase

What is enzyme for Urea?

urease

What is enzyme for SIM

cystein desulfhydrase theosulfate reductase, trytophonase

What is product for Lysine?

Alk cadavarine

What is product for phenylalanine?

ammonia phenylpruvic acid

What is product for Urea?

ammonia

What is product for SIM

H2S, indole ammonia

What is indicator for lysine?

Bromecresol purple

What is phenylalanine indicator?

ferric chloride FeCl3

What is Urea indicator?

pH phenol red

What is indicator for SIM?

Iron sulfate FeSO4, Kovac’s reagent not pH

What is positive lysine?

purple

What is positive phenylalanine?

green

What is positive Urea?

pink pp, only slant pink p

what is positive Sulfur?

black H2S

What is positive Indole?

red ring

What is Motility positive?

3-D cloud 1 flagella

What is Motility negative?

2-D cloud