Tumor imaging and tracking, therapeutic potential of hippo yap taz

0.0(0)
Studied by 0 people
call kaiCall Kai
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/8

encourage image

There's no tags or description

Looks like no tags are added yet.

9 Terms

1
New cards

Definition of transgenic mouse model

  • mice genetically engineered ro develop cancer from normal tissue


2
New cards

4 different types of transplant mouse models

  • Syngeneic: Mouse cancer cells injected into a genetically identical mouse

  • Allograft: Mouse cancer cells injected into a mouse- sometimes used interchangeable with Syngeneic, but could also be mouse cells injected into non-genetically identical immunocompromised mouse

  • Xenograft: non-mouse cancer cells injected into an immunocompromised (usually) or humanized mouse

  • Patient Derived Xenograft (PDX): Cells taken from a cancer patient and directly injected into a immunocompromised (usually) or humanized mouse.


3
New cards

Injection sites

  • Orthotopic: Cancer cells injected into the same organ/tissue from which the cancer originated

  • Subcutaneous: Cancer cells injected under the skin

  • Intravenous (tail vein, retro-orbital): Cancer cells injected into venous circulation (will return to heart -> then the lungs)

  • Arterial (intracardiac): Cells injected directly into left ventricle so they enter systemic circulation

  • Direct injection into non-orthoptic organ- Used to model metastatic colonization of specific tissues (intratibial, portal vein, intracranial, ect.)


4
New cards

Experimental metastasis assay (2)

  • inject cells intravenously into the tail vain

    • assay lung metasteses and extravasation

  • intracardiac injections

    • assay systemic metastesis


5
New cards

Image of the metastatic cascade

knowt flashcard image
6
New cards

which model can be used to label tumor cells

knowt flashcard image
7
New cards

What enzyme can you have in your cancer cells to allow you to assay tumor and metastatic growth over time in living mice?

Luciferase

<p>Luciferase</p>
8
New cards

Diagram showing how to assay each step of the metastatic cascade

knowt flashcard image
9
New cards

How can you use intracardiac injections to assess tropism or tissue specific requirement of a gene

  • have luciferase expressing cells

  • inject into heart

  • DTC seeding wull occur

  • induce RNAi/drug

  • quantify metastatic burden over time (IVIS)

    • specifically, you can look at diff orans/tisues to see if cells are growing there (brain, lungs, liver, bone)


<ul><li><p>have luciferase expressing cells</p></li><li><p>inject into heart</p></li><li><p>DTC seeding wull occur</p></li><li><p>induce RNAi/drug</p></li><li><p>quantify metastatic burden over time (IVIS)</p><ul><li><p>specifically, you can look at diff orans/tisues to see if cells are growing there (brain, lungs, liver, bone)</p></li></ul></li></ul><p></p>