Cervical and Vaginal Cytology

Conventional Smear Process

• Spatula rotated 360 degrees on vaginal wall

• Brush rotated 90 degrees in EC canal

• Slide and then Brush rolled over slide

ThinPrep Process

• Sampling device swirled in ethanol solution

• Cells passed through a filter

• Cells stamped into slide

Sure Path Process

• Uses ethanol based transport medium

• Sampling device vortexed and cells disaggregate

• Cells transfered to sediment tubed and RBCs removed

• Cells stamped onto slide

ThinPrep Imagine System (TIS)

• Can scan 300 slides per hour

• Shows 22 FOVs

• If anything atypical, then rescreen slide

BD FocalPoint Guided Screening Imaging System

Measures cells using cytologic criteria and rates them in a score from 0-1.0

SAT Requirements for PAP Smear

5,000 cells for liquid based and 10,000 for conventional smears. Count 10 fields of view are 40x magnification

Superficial Cells