Cell and Molecular BIO Exam 3 - Questionable topics

protein responsible for pinching off vesicle during transport, requires ___ hydrolysis

dynamin, GTP

three-legged structure formed by clathrin

triskelion

necessary addition for lysosomal transport

mannose-6-phosphate

receptor mediated endocytosis is associated with ______ coated vesicles

clathrin

pH dependent association molecules

mannose-6-phosphate, LDL

sequence necessary for retrograde transport

KDEL

GPCR complete pathway for Gas (9)

Epinephrine, GPCR, trimeric G protein, GTP Ga activation, adenylyl cyclase, cAMP, pKa, glycogen, glucose, ATP

GPCR complete pathway for Gaq

Epinephrine, GPCR, trimeric G protein, GTP activation of Ga, phospholipase C, IP3 and DAG, Ca+ release and membrane messenging, pKc, proliferation

Describe the other pathway of GPCR involving Gaq and IP3 specifically (3 steps after Ca+ release)

IP3 triggers NO production, pKg stimulation, relaxes muscle tissue, produces cGMP

GTPase main function

hydrolyze GTP to GDP

What method to visualize GPCR and why?

FRET, can observe trimeric dimerization

How does phospholipase C form IP3 and DAP

phosphorylation of PI into PIP2, cleavage of PIP2 into products

TGF B signaling pathway

TGF B binds to three different receptors, receptors phosphorylate SMAD, SMAD regulates transcription—acting as tumor suppressor

Two inhibitors of SMAD (TGF B)

HDAT, Ski

Cytokine receptor pathway example

Kidney produce Epo, Epo binds to EpoR, EpoR recruits JAK, JAK phosphorylates STAT, STAT upregulates erythrocyte maturation

Tyrosine receptor kinase pathway example (7)

FGF binds to FGFR, GRB2, SOS, Ras, Raf, MapK, proliferation

regulates FGF and FGFR binding

proteoglycan with heparin sulfate group

Wnt pathway

Wnt ligand, frizzled receptor, b catenin TF

Hedgehog pathway

Hedgehog ligand, patch receptor, Ci TF

TNFa pathway

TNFa ligand, TNFa receptor, NFkB TF

Notch pathway

Delta ligand, Notch receptor, notch fragment TF

Features shared by GPCR, TGF B, RTK, Cytokine

phosphorylation

Features shared by Hedgehog, TNFa, Notch, Wnt

disassembly of complexes

Which AA is phosphorylated by JAK

Tyrosine