culture media (masterize)

Saboraud’s dextrose agar

only fungi are allowed to grow (pH of 5.6)

Thayer-martin media chocolate agar

neisseria gonorrhoaeae

Lowenstein-jenssen media

mycobacterium tuberculosis

“very selective, expensive”

Loeffler’s serum media (tellurite media)

Corynebacteruum diphtheria

Campy BAP (skirrow’s agar)

Campylobacter jejuni

Bordet-gengou agar

bordetella pertussis

Colistin - nalidixic acid

G(+) microorganisms

Thiosulfate-citrate Bile salt

Vibrio cholera

Charcoal yest agar

Legionella pneumophila

Barbour-stoenner-kelly media

Borrelia burgdorgeri

Eaton’s agar

Mycoplasma pneumonia

Cetrimide agar

Pseydomonas aeruginosa

Xyline-lysine deoxycholate (XLD) agar

Shigella / Salmonella

Fletcher’s media

Leptospira interrogans

Egg yolk agar

Clostridium perfingens

Chocolate agar + Factor X, V

Haemophilus

Mannitol-salt agar

Staphylococcus

Anaerobic Media

Liquid Media

• Thioglycolic acid

• Reduce oxygen present

Solid Media

• Candle Jar

• Gas-Pak ®

Simple Media

• For nonfastidious microorganisms; do not require special nutrition

• Nutrient agar, nutrient broth

Enriched Media

For fastidious MO (with special requirement)

Contain special vitamins and minerals

• Blood Agar Plate (BAP)

• Chocolate Agar

• Milk Agar

Blood Agar Plate (BAP)

↑ nucleic acid nutrition

• Hemolytic organisms (Haemophilus, Streptococcus)

• 5% sheep blood

Chocolate Agar

↑ Hgb nutrition

Milk Agar

↑ protein nutrition

MacConkey Agar

• LF: Pink (E. coli, K. pneumoniae)

• NLF: Colorless (Salmonella, Shigella, Proteus sp.)

Eosin Methylene Blue

• LF: Metallic green or pink (E.coli metallic green, some Enterobacter pink)

• NLF: Colorless or faint pink

Rapid Lactose Fermenters

• Escherichia

• Klebsiella

• Enterobacter

Slow Lactose Fermenters

• Serratia

• Citrobacter

• Arizona

• Providencia

• Edwardsiella

• Erwinia

• Hafnia

Non-Lactose Fermenters

• Salmonella (Motile)

• Shigella (Non-motile)

• Proteus

• Pseudomonas

Blood Agar Plate

• Blood enriched and differential

• Subdivides Streptococcus into different hemolytic reactions

Alpha-Hemolytic

• Partial Hemolysis;

• VR: Green

• Streptococcus viridans

• Streptococcus pneumoniae

Beta-Hemolytic

• Complete hemolysis;

• VR: Colorless/yellow

• Most virulent

• Lancefield Classification

• Group A: Streptococcus pyogenes

• Group B: Streptococcus agalactiae

Gamma-Hemolytic

• Cannot hemolyze;

• VR: No change, red agar in the background

• Enterococcus

Mannitol-Salt Agar

• Classifies Staphylococcus based on color reactions

Staphylococcus aureus

Golden-yellow colonies against yellow agar

Staphylococcus epidermidis

Porcelain red, pink colonies against red agar

Staphylococcus saprophyticus

May turn slightly yellow or stay pink, weak mannitol fermenter