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cell fractionation
technique that breaks cells and separates their components by serial centrifugation
cytoplasm
aqueous interior of the cell within which particles and organelles are suspended

nucleoid
densly staining DNA region in a prokaryotic cell

chromatin
protein and DNA complex
chromosome
single molecule of DNA; contains hereditary information

nucleus
organelle that contains DNA and controls processes of cell

nucleolus
organelle where ribosomes are synthesized and partially assembled (within nucleus)

nuclear envelope
perforated double membrane that controls flow of materials (around nucleus)

ribosomes
organelles made of protein and rRNA that direct protein synthesis (in cytoplasm or tethered to rough ER)

nuclear lamina
netlike array of intermediate filaments that protects and maintains shape of nucleus
endoplasmic reticulum (ER)
internal membrane system in which components of cell membrane and some proteins are constructed
rough ER
organelle continuoues with nuclear membrane, to which ribosomes are tethered and proteins are synthesized for export

smooth ER
organelle where lipids are synthesized

vesicles
small sacs surrounded by phospholipid bilayer that specialize in moving products into, out of, and within a cell

golgi apparatus
stack of membranes that modifies, sorts, and packages proteins for transport

lysosome
organelle filled with enzymes that break down chemicals

phagocytosis
when extensions of cytoplasm surround and engulf large food particles

central vacuole
membranous sac in plant cells with roles in reproduction, growth, and storage

mitochondria
organelle in all eukaryotic cells where chemical energy in glucose and fats is transformed into ATP (cellular respiration SITE)

chloroplast
organelle in plants where sunlight energy is used to synthesize glucose (photosynthesis SITE)

endosymbiont theory
states that mitochondria and chloroplasts originated as prokaryotic cells engulfed by ancestral eukaryotic cell
mitochondrial matrix
innermost compartment of the mitochondria; Krebs cycle occurs here

cristae
inner membrane infoldinfgs of mitochondria that increase surface area (Electron Transport Chain and ATP synthase are located here)

thylakoids
flattened membrane sacs in chloroplast (contains proteins for light-dependent reactions in photosynthesis)

grana
stacked thylakoid membranes in chloroplast

stroma
innermost compartment of chloroplast (Calvin cycle or light-independent reactions occur here)

peroxisomes
animal orgnalles that contain enzymes that detoxify alcohols, hydrogen peroxide, and other harmful chemicals

cytoskeleton
microscopic network of protein fibers within cytoplasm that gives shape to cell

microtubules
(bottom) thickest, hollow tubes of tubulin proteins that make up cilia, flagella, and mitotic spindle and help move organelles around cell

microfilaments
(top) thinnest, intertwined fibers of actin proteins that allow cytoplasmic streaming, ameboid movement and keep cell extensions rigid (microvilli, axons)

intermediate filaments
(middle) medium, coiled fibers of keratin that help maintain cell shape, stabilize positions of organelles

centrioles
organelles in animal cells from which mitotic spindle forms during cell division (resemble basal bodies)

mitotic spindle
microtubules that attach to centromeres through kinetochore proteins of chromosomes and pull sister chromatids apart during cell divisions

cilia
(A) short, many extensions of membrane-covered microtubules that move cell or materials past cell (ex. paramecium, trachea)

flagella
(E) long, few, whiplike tails of membrane-covered microtubules that move cells

cytoplasmic streaming
circular flow of cytosol using myosin and actin filaments that speeds up distribution of materials within cells
cell wall
strong supporting layer around the cell membrane. Composed of peptidoglycan (prokaryotes), chitin (fungi) or cellulose (plants)

extracellular matrix (ECM)
substance in which animal cells are embedded, consisting of proteins and polysaccharides

plasmodesmata
(2) openings in plant cell walls through which strands of ER and cytosol connect adjacent cells

gap junctions
(C) cytoplasmic channels between adjacent animal cells that allow movement of materials

tight junctions
(A) membrane fusions of neighboring cells that prevent leakage of fluid between them

adhering junctions
(B) anchoring junctions that prevents cells subjected to mechanical stress from being pulled apart

prokaryote
unicellular organism without nucleus and membrane bound organelles (archea and bacteria)

eukaryote
cells with nucleus and membrane bound organelles

psuedopodia
temporary projections of eukaryotic cell membranes or unicellular protists in direction of movement
collagen
fibrous protein found in connective tissues and ECM