Microbiology Exam 2
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22 Terms
E. coli
Colony Morphology: dark lactose fermenter, beta hemolytic
Indole: Positive
Motile
Common Clinical Scenario: UTI, bactremia
O157 has Shiga-toxin like gene
Proteus vulgaris
Colony Morphology: Swarming, chocolate cake smell, non-lactose fermenter
Indole: Positive
Urea Hydrolysis: Positive
Common Clinical Scenario: UTI
Proteus mirabilis
Colony Morphology: Swarming, chocolate cake smell, non-lactose fermenter
Indole: Negative
Urea Hydrolysis: Positive
Common Clinical Scenario: UTI
Klebsiella oxytoca
Colony Morphology: Mucoid lactose fermenter
Indole: Positive
Common Clinical Scenario: Pneumonia
High rate of plasmid mediated drug resistance
Klebsiella pneumoniae
Colony Morphology: Mucoid lactose fermenter
Indole: Negative
Common Clinical Scenario: Pneumonia
High rate of plasmid mediated drug resistance
Enterobacter cloacae
Colony Morphology: Mucoid lactose fermenter
Indole: Negative
Motile
Inherent resistance to ampicillin, 1st and 2nd generation cephalosporins
Klebsiella(Enterobacter) aerogenes
Colony Morphology: Mucoid lactose fermenters
Indole: Negative
Motile
Inherent resistance to ampicillin, 1st and 2nd generation cephalosporins
Serratia marcescens
Colony Morphology: Non-lactose fermenter, can have red pigment
Indole: Negative
Motile
Common Clinical Scenario: Hospital acquired infection, medical device infections
CItrobacter freundii
Colony Morphology: Non-lactose fermenter
Indole: Negative
H2S: Positive
Inherent resistance to ampicillin, 1st and 2nd generation cephalosporins
CItrobacter koseri
Colony Morphology: Non-lactose fermenter
Indole: Positive
H2S: Negative
Inherent resistance to ampicillin, 1st and 2nd generation cephalosporins
Salmonella
Colony Morphology: Non-lactose fermenter
HE Media: Black colonies
Indole: Negative
H2S: Positive
Motile
Common Clinical Scenario: GI illness, food borne
Shigella
Colony Morphology: Non-lactose fermenter
HE Media: Green/Teal colonies
Indole: Negative
Non-motile
Common Clinical Scenario: GI illness, person to person
Yersinia
Colony Morphology: Non-lactose fermenter
Growth on CIN Agar
Urea Hydrolysis: Positive
Common Clinical Scenario: GI illness, bubonic plague(Yersinia pestis), pseudoappendicitis
Pseduomonas aeruginosa
Gram neg rods
Colony Morphology: spreading,flat colony with metallic sheen, Beta hemolytic, mucoid
Growth on MAC
Grape-like odor
Aerobic
Motile w/Flagellum
Catalas: Positive
Oxidase: Positive
Cytotoxicity: Protease, exotoxin A, & phospholipases
Community or healthcare acquired
Burkholderia
Grows on routine media (BAP & CHOC)
MAC Growth
B.cepacia agar(BCSA): red/orange color from pyruvate breakdown
Acinetobacter
Gram negative coccobacilli
Colony morphology: Smooth opaque raised creamy colonies on BAP
Growth on MAC: nonlactose fermenter with purple hue
Oxidase: Negative
Non-motile
Nitrate: Negative
Catalase: Positive
Strenotrophomonas
Gram negative short to medium-size straight rods
Oxidase: Negative
Glucose Oxidizer
Vibrio
Aeromonas
Plesiomonas
Campylobacter
Helicobacter