BIO 112: LAB PCR and DNA Isolation

PCR

the process that amplifies a specific segment of DNA creating a multitude of copies using cycles of heating and cooling,

DNA Isolation

The process of separating DNA from a cellular component using a multitude of steps to remove proteins

denaturation

The pcr step that is made to separate the 2 double helix strands of the DNA at 94 °C

annealing

the pcr step that is done at 55 °C where forward and backward primers bind to a specific DNA sequence

extension

the pcr step that uses to synthesize the new dna and is at 72C

70% ethanol

The substance used to used as a washing agent to clean the precipitated DNA pellet

isopropanol

The substance that acts as a precipitating agent that forces DNA out of an aqueous (water-based) solution so it can be collected as a solid pellet.

centrifuging

The equipment that separate cellular components, proteins, and debris from DNA based on differences in size and density

buffers

The substance that break open cells, maintain a stable pH, and protect the genetic material from degradation

vortex

The equipment that rapidly mixes substances

COI

The Invertebrate gene that is used during pcr

rbcl

18s and 28s RNA