Exercise 6: Cholesterol Measurement

Cholesterol Measurement Review

It is an unsaturated sterol alcohol present in all cells and an integral component of the cell membrane

Cholesterol

Give the steroid hormones in cholesterol

(Format: mama, papa, ate, kuya, bunsong makulit)

cortisol, estrogen, testosterone, progesterone, bile salts

A form of cholesterol that is amphipathic and found with phospholipids in the bilayer membrane of cells.

Free cholesterol

A form of cholesterol that is hydrophobic and needs to be carried by low density lipoprotein (LDL-C).

Cholesteryl esters

What are the diseases that is strongly associated with serum cholesterol concentration, particulate LDL-C?

(Format: xxxx yyyyy, zzzzz)

cardiovascular disease, atherosclerosis

It is known as the bad cholesterol and if increased, it can be alarming.

Low density lipoprotein

It is known as the good cholesterol and it is good if increased.

High density lipoprotein

Give 4 patient preparation for cholesterol.

(Format: blahblahblah, blehbleh blehbleh, blihblihblih blihblih, blohbloh blohblohbloh)

*IF TAMA NAMAN SAGOT MO, CONSIDERED PA RIN AS CORRECT AS LONG AS RELATED DUN SA APAT NA YUN*

No fasting required, avoid smoking and strenuous exercise prior to collection, let the patient be seated at least 5 minutes, tourniquet application

It is the most common used specimen.

Serum

True or False: plasma can also be used in cholesterol measurement.

True

Give the plasma tube used in clinical chemistry laboratory?

(Format: amen/lord)

*KAHIT BALIGTAD, CONSIDERED AS TAMA PA RIN*

Purple/lavender

What is the interference of cholesterol measurement?

Hemolyzed sample

Enumerate the rejection criteria for cholesterol. (In any order)

(Format: pagod na ako, sa course na to, ipasa mo na ako, kahit tres lang oh)

*PAG MINALI KAYO, CONSIDERED AS TAMA PA RIN AS LONG AS NANDUN YUNG SAGOT*

Collection tube used is wrong, improperly labeled specimen, quantity not sufficient, no requisition form, hemolyzed sample

Identify the reaction:

Cholesterol esterase

Identify the reaction:

Peroxidase

Identify the reaction:

Cholesterol oxidase

What do you call on a cholesterol present in serum? (In any order)

(Format: xxx zz and yyy aa)

*KAHIT ANO YUNG AYOS MO DUN SA DALAWA, CONSIDERED AS CORRECT PA DIN*

Cholesterol esters and free cholesterol

Cholesterol esters present in serum is hydrolyzed by?

Cholesterol esterase

Cholesterol is measured by oxidizing with?

Cholesterol oxidase

The cholesterol is oxidized and forms?

Hydrogen peroxide

What are the 2 substances that hydrogen peroxide reacts to form the colored dye?

(Format: xx and y-zzzz)

Phenol and 4-aminoantipyrine

End color product of cholesterol measurement?

Red

What is the dye used in cholesterol?

Red quinonimine dye

Relationship of intensity of dye to level of cholesterol present?

Directly proportional

Volume of reagent?

1000uL

Volume of distilled water and sample to be added?

10uL

Incubation period for cholesterol?

*KAHIT ANO DUN SA DALAWA, TAMA KA PA RIN*

10 minutes at 15 - 25C in waterbath or 5 minutes at 37C

How long does cholesterol can be stable?

60 minutes

What is the wavelength for cholesterol measurement?

500nm

What is the conversion factor from conventional to SI unit?

0\.026

What is the desirable state of cholesterol?

What is the borderline high reference range?

200 - 239mg/dL

What is the high cholesterol state?

*KAHIT HINDI NA YUNG MISMONG SIGN ANG ILAGAY, TAMA PA DIN*

≥240mg/dL

What is the multiplication factor for cholesterol?

200mg/dL

What is the main cause of prolonged tourniquet application in cholesterol concentration? (Answer: falsely decrease or falsely increase)

Falsely increase

Give the 2 sources of cholesterol.

(Format: mhiema and teh)

Dietary and Endogenous

What is the method used for measuring a cholesterol? (Include the ‘method’ word)

Cholesterol oxidase method

Conventional unit for Cholesterol.

mg/dL

SI unit for Cholesterol.

mmol/L

What type of bond is present in Cholesterol Measurement?

Ester Bond