7. Formula sheet & Inhibition pattern

Dilution:

C₁V₁ = C₂V₂

Beer–Lambert law:

A = εlc

Rate from absorbance:

Δc/Δt = (ΔA/Δt)/(εl)

Gel-filtration distribution coefficient:

Ka = (Ve − Vo)/(Vt − Vo)

Gel-filtration calibration:

Use the standard-curve line to estimate log₁₀(MW), then MW = 10^(log₁₀ MW)

Michaelis–Menten:

v₀ = Vmax[S]/(Km + [S])

Maximum velocity:

Vmax = kcat × ET

Turnover number:

kcat = Vmax/ET

Lineweaver–Burk:

1/v₀ = (Km/Vmax)(1/[S]) + 1/Vmax

Lineweaver–Burk intercepts:

y-intercept = 1/Vmax; x-intercept = −1/Km; slope = Km/Vmax

Inhibition Pattern Type: Competitive

Describe

1. Binding

2. Apparent Km

3. Apparent Vmax

1. Binding: Free E/ active site; competes with S

2. Apparent Km: Increases

3. Apparent Vmax: Unchanged

Inhibition Pattern Type: Uncompetitive

Describe

1. Binding

2. Apparent Km

3. Apparent Vmax

1. Binding: ES only

2. Apparent Km: Decreases

3. Apparent Vmax: Decreases

Inhibition Pattern Type: Pure noncompetitive

Describe

1. Binding

2. Apparent Km

3. Apparent Vmax

1. Binding: E and ES equally

2. Apparent Km: Unchanged

3. Apparent Vmax: Decreases

Inhibition Pattern Type: Mixed

Describe

1. Binding

2. Apparent Km

3. Apparent Vmax

1. Binding: E and ES with unequal affinities

2. Apparent Km: Can increase or decrease

3. Apparent Vmax: Decreases